AB151484

Anti-CysLT1 antibody - N-terminal

Name: Anti-CysLT1 antibody - N-terminal (ab151484) | Abcam
Brand: Abcam Limited
SKU: AB151484
Price: 535 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal CysLT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CYSLTR1 aa 1-50.

View Alternative Names

CYSLT1, CYSLTR1, Cysteinyl leukotriene receptor 1, CysLTR1, Cysteinyl leukotriene D4 receptor, G-protein coupled receptor HG55, HMTMF81, LTD4 receptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CysLT1 antibody - N-terminal (AB151484)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CysLT1 antibody - N-terminal (AB151484)

Immunohistochemical analysis of paraffin embedded U373 xenograft labeling CysLT1 with ab151484 antibody at 1/100.

Supplier Data

Western blot - Anti-CysLT1 antibody - N-terminal (AB151484)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Anti-CysLT1 antibody - N-terminal (ab151484) diluted at 1/500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CysLT1 antibody - N-terminal (ab151484)

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CYSLTR1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y271

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

See full target information CYSLTR1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 65:15 PubMed39504050

2024

Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Awad,Amritha T M Seetharaman,Mohammad Shahadat Hossain,Sally L Elshaer,Rania R Abdelaziz,Manar A Nader,Rajashekhar Gangaraju

Scientific reports 13:13239 PubMed37580467

2023

Inhibition of CysLTR1 reduces the levels of aggregated proteins in retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Koller,Susanne Maria Brunner,Julia Preishuber-Pflügl,Daniela Mayr,Anja-Maria Ladek,Christian Runge,Herbert Anton Reitsamer,Andrea Trost

Aging 13:25670-25693 PubMed34919533

2021

Chronobiological activity of cysteinyl leukotriene receptor 1 during basal and induced autophagy in the ARPE-19 retinal pigment epithelial cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Koller,Julia Preishuber-Pflügl,Christian Runge,Anja-Maria Ladek,Susanne Maria Brunner,Ludwig Aigner,Herbert Reitsamer,Andrea Trost

Scientific reports 10:17659 PubMed33077798

2020

Cysteinyl leukotriene receptor 1 modulates autophagic activity in retinal pigment epithelial cells.

Applications

WB, ICC

Species

Human, Human

Andreas Koller,Daniela Bruckner,Ludwig Aigner,Herbert Reitsamer,Andrea Trost

Cancers 12: PubMed33066024

2020

High Cysteinyl Leukotriene Receptor 1 Expression Correlates with Poor Survival of Uveal Melanoma Patients and Cognate Antagonist Drugs Modulate the Growth, Cancer Secretome, and Metabolism of Uveal Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kayleigh Slater,Aisling B Heeran,Sandra Garcia-Mulero,Helen Kalirai,Rebeca Sanz-Pamplona,Arman Rahman,Nebras Al-Attar,Mays Helmi,Fiona O'Connell,Rosa Bosch,Anna Portela,Alberto Villanueva,William M Gallagher,Lasse D Jensen,Josep M Piulats,Sarah E Coupland,Jacintha O'Sullivan,Breandán N Kennedy

Canadian respiratory journal 2019:7930396 PubMed31781316

2019

Tetrandrine Ameliorates Airway Remodeling of Chronic Asthma by Interfering TGF-1/Nrf-2/HO-1 Signaling Pathway-Mediated Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yiping Lin,Jingchan Yao,Meiling Wu,Xiaoqian Ying,Mingxing Ding,Yanli Wei,Xiaoyan Fu,Wei Feng,Yunguang Wang

The Journal of biological chemistry 292:3552-3567 PubMed28035003

2016

A Quininib Analogue and Cysteinyl Leukotriene Receptor Antagonist Inhibits Vascular Endothelial Growth Factor (VEGF)-independent Angiogenesis and Exerts an Additive Antiangiogenic Response with Bevacizumab.

Applications

Unspecified application

Species

Unspecified reactive species

Clare T Butler,Alison L Reynolds,Miriam Tosetto,Eugene T Dillon,Patrick J Guiry,Gerard Cagney,Jacintha O'Sullivan,Breandán N Kennedy

The Journal of biological chemistry 291:7242-55 PubMed26846851

2016

Phenotype-based Discovery of 2-[(E)-2-(Quinolin-2-yl)vinyl]phenol as a Novel Regulator of Ocular Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alison L Reynolds,Yolanda Alvarez,Temitope Sasore,Nora Waghorne,Clare T Butler,Claire Kilty,Andrew J Smith,Carmel McVicar,Vickie H Y Wong,Orla Galvin,Stephanie Merrigan,Janina Osman,Gleb Grebnev,Anita Sjölander,Alan W Stitt,Breandán N Kennedy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CysLT1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CysLT1 antibody - N-terminal (AB151484)

Western blot - Anti-CysLT1 antibody - N-terminal (AB151484)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise