Anti-Cystathionase/CTH antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystathionase/CTH antibody (AB151769)

Immunohistochemical analysis of paraffin embedded human hepatoma tissue labeling Cystathionase/CTH with ab151769 antibody at 1/500.

• WB

Supplier Data

Western blot - Anti-Cystathionase/CTH antibody (AB151769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Lab

Western blot - Anti-Cystathionase/CTH antibody (AB151769)

Western blot : Anti-CTH antibody (ab151769) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab151769 was shown to bind specifically to CTH. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CTH knockout cell line. To generate this image, wild-type and CTH knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CTH knockout A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-Cystathionase/CTH antibody (AB151769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cystathionase/CTH antibody (ab151769) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysates at 50 µg

Lane 2:

Mouse cervix tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-Cystathionase/CTH antibody (AB151769)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Cystathionase/CTH antibody (ab151769) at 1/3000 dilution

All lanes:

Rat liver tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 45 kDa

false