Rabbit Recombinant Monoclonal Cystatin C antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 27 publications.
Images
![Western blot - Anti-Cystatin C antibody [EPR4413] (AB109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img58697.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] (AB109508)")
![Western blot - Anti-Cystatin C antibody [EPR4413] (AB109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img116645.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] (AB109508)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61702.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400228.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508)")
Publications
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- International journal of molecular sciences 24:2023Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinying Jiang,Hailing Zhang,Hengshuo Zhang,Fan Wang,Xiaochang Wang,Tong Ding,Xuxiang Zhang,Ting WangPubMed 37445705
- Cells 12:2023Analysis of Wild Type and Variant B Cystatin C Interactome in Retinal Pigment Epithelium Cells Reveals Variant B Interacting Mitochondrial Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmil Carlsson,Umar Sharif,Wasu Supharattanasitthi,Luminita ParaoanPubMed 36899848
- Breast cancer research : BCR 25:212023Transcriptomic intratumor heterogeneity of breast cancer patient-derived organoids may reflect the unique biological features of the tumor of origin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumito Saeki,Kohei Kumegawa,Yoko Takahashi,Liying Yang,Tomo Osako,Mahmut Yasen,Kazutaka Otsuji,Kenichi Miyata,Kaoru Yamakawa,Jun Suzuka,Yuri Sakimoto,Yukinori Ozaki,Toshimi Takano,Takeshi Sano,Tetsuo Noda,Shinji Ohno,Ryoji Yao,Takayuki Ueno,Reo MaruyamaPubMed 36810117
- Frontiers in physiology 13:9509602022Wnt 3a Protects Myocardial Injury in Elderly Acute Myocardial Infarction by Inhibiting Serum Cystatin C/ROS-Induced Mitochondrial Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Shen,Ying Li,Yang Jiao,Jihang Wang,Xiaoling Hou,Yongkang Su,Bing Liu,Henan Liu,Zhijun Sun,Qing Xi,Zhenhong FuPubMed 35936906
- Journal of neuroinflammation 19:772022Proteomic landscape subtype and clinical prognosis of patients with the cognitive impairment by Japanese encephalitis infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Yin,Linpeng Yang,Ying Hao,Zhiqi Yang,Tao Lu,Wanjun Jin,Meiling Dan,Liang Peng,Yingjie Zhang,Yaxuan Wei,Rong Li,Huiping Ma,Yuanyuan Shi,Pengcheng FanPubMed 35379280
- Theranostics 11:5010-50272021Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Wang,Yanwen Xu,Yuncong Zhang,Yuhan Chen,Gang Chang,Geng An,Xinyan Yang,Caihong Zheng,Jiexiang Zhao,Zhaoting Liu,Dazhuang Wang,Kai Miao,Shuan Rao,Meng Dai,Dong Wang,Xiao-Yang ZhaoPubMed 33754041
- Annals of translational medicine 8:15142020Pressure overload promotes cystatin C secretion of cardiomyocytes to regulate the MAPK signaling pathway and mediate cardiac hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Shen,Xiaoyi Zhang,Chenguang Li,Xiang Wang,Yong Ye,Jie Yuan,Hui Gong,Yunzeng Zou,Junbo GePubMed 33313259
- RNA (New York, N.Y.) 27:190-2012020Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Indacochea,Santiago Guerrero,Macarena Ureña,Ferrán Araujo,Olga Coll,Matilde E LLeonart,Fátima GebauerPubMed 33172965
- Cell reports 32:1079542020Proximal Tubule mTORC1 Is a Central Player in the Pathophysiology of Diabetic Nephropathy and Its Correction by SGLT2 Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAviram Kogot-Levin,Liad Hinden,Yael Riahi,Tal Israeli,Boaz Tirosh,Erol Cerasi,Ernesto Bernal Mizrachi,Joseph Tam,Ofri Mosenzon,Gil LeibowitzPubMed 32726619
- Nature communications 11:35592020Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian Strunz et. al.PubMed 32678092
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/8000 | Notes - |
Species Mouse | Dilution info 1/250 - 1/8000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 - 1/8000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Alternative names
Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Recommended products
Rabbit Recombinant Monoclonal Cystatin C antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 27 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4413
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img58697.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: CST3 knockout HAP1 whole cell lysate (20 μg)
Lane 3: Caco2 whole cell lysate (20 μg)
Lane 4: U20S whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. ab109508 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
Predicted band size: 15 kDa, 19 kDa
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img116645.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
All lanes: Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution
Lane 1: Human testis lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: U87-MG cell lysate at 10 µg
Predicted band size: 15 kDa
Observed band size: 13 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61702.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400228.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)
Immunohistochemistry analysis of paraffin-embedded rat kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on rat kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)
Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on mouse kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)
Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labelling Cystatin C with ab109508 at 1/8000 dilution. The section was incubated with ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on human kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img400225.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBSTLane 1: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (Anti-Cystatin C antibody [EPR4413] - BSA and Azide free ab217569) at 1/10000 dilution
Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution
All lanes: Mouse thymus tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa
Exposure time: 3min
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img400224.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBSTLane 1: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (Anti-Cystatin C antibody [EPR4413] - BSA and Azide free ab217569) at 1/10000 dilution
Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution
All lanes: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa
Exposure time: 80s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)")
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img400225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
![Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-ab109508--western-blot-img400224.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)")
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