Rabbit Recombinant Monoclonal Cystatin C antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img185325.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)")
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img176732.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61702.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)")
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img400225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)")
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img400224.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (AB217569)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Alternative names
Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Recommended products
Rabbit Recombinant Monoclonal Cystatin C antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Alternative names
Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4413
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab217569 is the carrier-free version of Anti-Cystatin C antibody [EPR4413] ab109508.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img185325.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: CST3 knockout HAP1 whole cell lysate (20 μg)
Lane 3: Caco2 whole cell lysate (20 μg)
Lane 4: U20S whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-Cystatin C antibody [EPR4413] ab109508 observed at 15 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Cystatin C antibody [EPR4413] ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. Anti-Cystatin C antibody [EPR4413] ab109508 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Predicted band size: 15 kDa
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img176732.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution
Lane 1: Human testis lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: U87-MG cell lysate at 10 µg
Predicted band size: 15 kDa
Observed band size: 13 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61702.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Immunohistochemical analysis of paraffin-embedded Human brain tissue using Anti-Cystatin C antibody [EPR4413] ab109508 at adilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cystatin C antibody [EPR4413] ab109508).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img400225.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution
Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] (Anti-Cystatin C antibody [EPR4413] ab109508) at 1/10000 dilution
All lanes: Mouse thymus tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa
Exposure time: 3min
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST![Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--western-blot-img400224.jpg "Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569) at 1/10000 dilution
Lane 1: Western blot - Anti-Cystatin C antibody [EPR4413] (Anti-Cystatin C antibody [EPR4413] ab109508) at 1/10000 dilution
All lanes: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 13 kDa
Exposure time: 80s
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400228.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Immunohistochemistry analysis of paraffin-embedded rat kidney tissue sections labelling Cystatin C with Anti-Cystatin C antibody [EPR4413] ab109508 at 1/8000 dilution. The section was incubated with Anti-Cystatin C antibody [EPR4413] ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on rat kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue sections labelling Cystatin C with Anti-Cystatin C antibody [EPR4413] ab109508 at 1/8000 dilution. The section was incubated with Anti-Cystatin C antibody [EPR4413] ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on mouse kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)
Immunohistochemistry analysis of paraffin-embedded human kidney tissue sections labelling Cystatin C with Anti-Cystatin C antibody [EPR4413] ab109508 at 1/8000 dilution. The section was incubated with Anti-Cystatin C antibody [EPR4413] ab109508 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on human kidney tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using Anti-Cystatin C antibody [EPR4413] ab109508, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400228.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569), expandable thumbnail](https://content.abcam.com/products/images/cystatin-c-antibody-epr4413-bsa-and-azide-free-ab217569--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] - BSA and Azide free (ab217569)")
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