AB133495

Anti-Cystatin C antibody [EPR4502]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Cystatin C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4502] (AB133495)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4502] (AB133495)

Immunohistochemical analysis of Cystatin C in paraffin embedded Human ovarian tissue using ab133495 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Cystatin C antibody [EPR4502] (AB133495)

All lanes:

Western blot - Anti-Cystatin C antibody [EPR4502] (ab133495) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human brain lysate at 10 µg

Lane 3:

PC3 lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 13 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4502

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

See full target information CST3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Antibiotics (Basel, Switzerland) 12: PubMed37107091

2023

Development of Chitosan Particles Loaded with siRNA for Cystatin C to Control Intracellular Drug-Resistant .

Applications

Unspecified application

Species

Unspecified reactive species

David Pires,Manoj Mandal,Ana I Matos,Carina Peres,Maria João Catalão,José Miguel Azevedo-Pereira,Ronit Satchi-Fainaro,Helena F Florindo,Elsa Anes

Clinical proteomics 16:34 PubMed31467500

2019

Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel.

Applications

Unspecified application

Species

Unspecified reactive species

Seong Beom Ahn,Samridhi Sharma,Abidali Mohamedali,Sadia Mahboob,William J Redmond,Dana Pascovici,Jemma X Wu,Thiri Zaw,Subash Adhikari,Vineet Vaibhav,Edouard C Nice,Mark S Baker

International journal of oncology 54:1086-1098 PubMed30628664

2019

Stathmin gene silencing suppresses proliferation, migration and invasion of gastric cancer cells via AKT/sCLU and STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Shu,Xiaoqin Zou,Huan Tuo,Sha She,Juan Huang,Hong Ren,Huaidong Hu,Shifang Peng,Jiandong Wang,Yixuan Yang

Molecular & cellular proteomics : MCP 15:1220-31 PubMed26711467

2015

Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Steffan Vartanian,Taylur P Ma,James Lee,Peter M Haverty,Donald S Kirkpatrick,Kebing Yu,David Stokoe

View all publications

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Product promise

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