Rabbit Recombinant Monoclonal Cystatin F antibody. Suitable for IHC-P, Flow Cyt (Intra), IP and reacts with Transfected cell line, Human samples.
Images
![Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (AB309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunoprecipitation-img416486.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (AB309544)")
![Flow Cytometry (Intracellular) - Anti-Cystatin F antibody [EPR28408-30] (AB309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--flow-cytometry-intracellular-img416625.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cystatin F antibody [EPR28408-30] (AB309544)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416551.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416549.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (AB309544)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | IP | ICC/IF | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line | Dilution info - | Notes - |
Target data
Function
Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Alternative names
Cystatin-F, Cystatin-7, Cystatin-like metastasis-associated protein, Leukocystatin, CMAP, CST7
Recommended products
Rabbit Recombinant Monoclonal Cystatin F antibody. Suitable for IHC-P, Flow Cyt (Intra), IP and reacts with Transfected cell line, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28408-30
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (ab309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunoprecipitation-img416486.jpg "Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (ab309544)")
Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (ab309544)
Cystatin F was immunoprecipitated from 0.35 mg HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate with ab309544 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lane 2: ab309544 IP in HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab309544 in HL-60 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 secondsAll lanes: Immunoprecipitation - Anti-Cystatin F antibody [EPR28408-30] (ab309544) at 1/30 dilution
All lanes: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Flow Cytometry (Intracellular) - Anti-Cystatin F antibody [EPR28408-30] (ab309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--flow-cytometry-intracellular-img416625.jpg "Flow Cytometry (Intracellular) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)")
Flow Cytometry (Intracellular) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labelling Cystatin F with ab309544 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: K-562.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416551.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Cystatin F with ab309544 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on HEK-293T transfected with a His-tagged CST7 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab309544 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cystatin F with ab309544 at 1/100 (5.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human cerebrum (PMID:28178353). The section was incubated with ab309544 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544), expandable thumbnail](https://content.abcam.com/products/images/cystatin-f-antibody-epr28408-30-ab309544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img416549.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin F antibody [EPR28408-30] (ab309544)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Cystatin F with ab309544 at 1/100 (5.14 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human spleen. The section was incubated with ab309544 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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