AB151771

Anti-Cystatin S antibody

Name: Anti-Cystatin S antibody (ab151771) | Abcam
Brand: Abcam Limited
SKU: AB151771

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(3 Publications)

Rabbit Polyclonal Cystatin S antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CST4 aa 1-100.

View Alternative Names

Cystatin-S, Cystatin-4, Cystatin-SA-III, Salivary acidic protein 1, CST4

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (AB151771)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (AB151771)

Immunofluorescent analysis of methanol-fixed A431 cells labeling Cystatin S with ab151771 at 1/200. Lower panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (AB151771)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (AB151771)

Immunohistochemical analysis of paraffin embedded SW480 xenograft, labeling Cystatin S with ab151771 antibody at 1/100.

Unknown

Western blot - Anti-Cystatin S antibody (AB151771)

All lanes:

Western blot - Anti-Cystatin S antibody (ab151771) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 16 kDa

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CiteAb

Western blot - Anti-Cystatin S antibody (AB151771)

Cystatin S western blot using anti-Cystatin S antibody ab151771. Publication image and figure legend from Odanaka, H., Obama, T., et al., 2020, Biol Res, PubMed 31941552.

ab151771 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab151771 please see the product overview.

Detection of pellicle proteins by western blot. Samples (5 μg/lane) from pellicle, GCF, parotid gland, and mixed gland, were subjected to SDS-PAGE. Three pellicle proteins, cystatin S (a), α-amylase (b), and serotransferrin (c), were examined by western blot

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CST4 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P01036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin S also known as CST4 is a cysteine protease inhibitor with a molecular mass of approximately 14 kDa. This protein belongs to the cystatin superfamily which is a group of proteins that inhibit proteases such as cathepsins. Cystatin S is mainly expressed in human saliva tears and other bodily fluids where it plays a significant role in protecting tissues from protease activity. Expression levels of cystatin S vary across tissues and can influence its inhibitory functions.

Biological function summary

Cystatin S regulates proteolytic activity by inhibiting enzymes like papain and cathepsins. This protein aids in maintaining the equilibrium between proteases and their inhibitors which is essential for protecting extracellular matrices. Cystatin S does not form part of a larger complex but functions independently to provide defense against unwanted proteolysis in the oral cavity and other environments.

Pathways

Cystatin S interacts with proteolytic pathways that handle extracellular matrix degradation and inflammatory responses. Inhibiting cathepsins within these pathways counters tissue destruction and contributes to cellular homeostasis. Cystatin-related proteins such as cystatin C share similar inhibitory roles indicating their importance in overlapping regulatory networks.

Aberrant expression or function of cystatin S associates with conditions like periodontitis and Sjögren's syndrome. In periodontitis imbalance in protease inhibition leads to periodontal tissue breakdown. Sjögren's syndrome characterized by reduced secretion of tears and saliva links to altered cystatin S levels and its interaction with other protease inhibitors like cystatin C. Both disorders highlight the significance of cystatin S in maintaining tissue integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

See full target information CST4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:60 PubMed39992672

2025

Untargeted Tear Proteomics in a Large South-Asian Cohort Reveals Inflammatory Signaling, ECM Remodeling, and Altered Metabolism in Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Ramaraj Kannan,Rohit Shetty,Trailokyanath Panigrahi,Siew Kwan Koh,Pooja Khamar,Vrushali Deshpande,Rudy M M A Nuijts,Marlies Gijs,Krishnatej Nishtala,Lei Zhou,Arkasubhra Ghosh

Biological research 53:3 PubMed31941552

2020

Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hibiki Odanaka,Takashi Obama,Naoko Sawada,Marika Sugano,Hiroyuki Itabe,Matsuo Yamamoto

Archives of oral biology 96:104-112 PubMed30219637

2018

Salivary proteome patterns of individuals exposed to High Altitude.

Applications

Unspecified application

Species

Unspecified reactive species

Shikha Jain,Yasmin Ahmad,Kalpana Bhargava

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cystatin S antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cystatin S antibody (AB151771)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin S antibody (AB151771)

Western blot - Anti-Cystatin S antibody (AB151771)

Western blot - Anti-Cystatin S antibody (AB151771)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise