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AB68329

Anti-Cystatin SN/CST1 antibody

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(4 Publications)

Mouse Polyclonal Cystatin SN/CST1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CST1.

View Alternative Names

Cystatin-SN, Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1, CST1

1 Images
Western blot - Anti-Cystatin SN/CST1 antibody (AB68329)
  • WB

Unknown

Western blot - Anti-Cystatin SN/CST1 antibody (AB68329)

All lanes:

Western blot - Anti-Cystatin SN/CST1 antibody (ab68329) at 1/500 dilution

Lane 1:

Cystatin SN/CST1 transfected 293T cell lysate at 25 µg

Lane 2:

non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 16 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CST1.

P01037

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin SN also known as CST1 is a cysteine protease inhibitor that belongs to the family of cystatins. This protein has a mass of approximately 16 kDa and is expressed primarily in human saliva as well as other tissues like the nasal mucosa and skin. Its main role involves binding to and inhibiting cathepsins and other cysteine protease enzymes preventing the breakdown of cellular and extracellular proteins. Such inhibition protects tissues from damage and helps maintain normal protein turnover.
Biological function summary

CST1 inhibits proteolytic enzymes that are involved in degrading proteins within cells and tissues. This protein is not part of a larger complex but instead exerts its function through one-on-one interactions with target proteases. Through its inhibitory actions CST1 plays a protective role in various bodily fluids and epithelial surfaces contributing to the body's barrier and immune defense mechanisms against pathogens and tissue damage.

Pathways

CST1 is engaged in the regulation of protease activity which impacts the broader proteolytic pathways that are important to cellular homeostasis. This regulation is linked to the lysosomal cathepsin family and pathways such as the protein metabolism pathway. Related proteins within these pathways include cathepsin L and cathepsin B which are also cysteine proteases that rely on precise regulation by cystatin family proteins like CST1 to prevent excessive protein degradation.

CST1 has associations with conditions like chronic rhinosinusitis and certain cancers. Chronic rhinosinusitis involves excessive inflammation and tissue damage a process where CST1's inhibition of proteases like cathepsin B is important. In cancer abnormal CST1 expression levels have been noted in gastric and colorectal cancers suggesting a connection with tumor growth and metastasis. In these contexts altered expression or function of CST1 may interact with other proteins such as cathepsin L influencing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
See full target information CST1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1216585 PubMed37575244

2023

Machine learning-based integration develops a neutrophil-derived signature for improving outcomes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Gong,Xiaodan Chen,Fahui Liu,Yuhua Cao

Brazilian journal of otorhinolaryngology 88 Suppl 4:S108-S116 PubMed35953439

2022

MicroRNA-375 inhibits laryngeal squamous cell carcinoma progression via targeting CST1.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Dai,Zuojun Xie,Qiming Yang,Zhuanglong Zhong,Chun Zhong,Yongliang Qiu

Journal of oncology 2022:6406943 PubMed35498540

2022

Role of the Long Intergenic Non-Protein-Coding RNA 1278/miR-185-5p/Cystatin SN Axis in Laryngeal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Shen,Haibin Ding,Yanyan Ye,Baozhen Luo

Cancer management and research 13:1901-1907 PubMed33658852

2021

CST1 Promoted Gastric Cancer Migration and Invasion Through Activating Wnt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Si Chen,Yingling Liu,Kaiguang Zhang,Lele Chen
View all publications
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