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Rabbit Recombinant Monoclonal Cystatin SN/CST1 antibody. Suitable for WB, IP and reacts with Human, Transfected cell line - Human samples.

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Images

Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (AB307416), expandable thumbnail
  • Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (AB307416), expandable thumbnail
  • Immunoprecipitation - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (AB307416), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow CytIP
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.

Alternative names

Cystatin-SN, Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1, CST1

Recommended products

Rabbit Recombinant Monoclonal Cystatin SN/CST1 antibody. Suitable for WB, IP and reacts with Human, Transfected cell line - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25933-111
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cystatin SN also known as CST1 is a cysteine protease inhibitor that belongs to the family of cystatins. This protein has a mass of approximately 16 kDa and is expressed primarily in human saliva as well as other tissues like the nasal mucosa and skin. Its main role involves binding to and inhibiting cathepsins and other cysteine protease enzymes preventing the breakdown of cellular and extracellular proteins. Such inhibition protects tissues from damage and helps maintain normal protein turnover.

Biological function summary

CST1 inhibits proteolytic enzymes that are involved in degrading proteins within cells and tissues. This protein is not part of a larger complex but instead exerts its function through one-on-one interactions with target proteases. Through its inhibitory actions CST1 plays a protective role in various bodily fluids and epithelial surfaces contributing to the body's barrier and immune defense mechanisms against pathogens and tissue damage.

Pathways

CST1 is engaged in the regulation of protease activity which impacts the broader proteolytic pathways that are important to cellular homeostasis. This regulation is linked to the lysosomal cathepsin family and pathways such as the protein metabolism pathway. Related proteins within these pathways include cathepsin L and cathepsin B which are also cysteine proteases that rely on precise regulation by cystatin family proteins like CST1 to prevent excessive protein degradation.

Associated diseases and disorders

CST1 has associations with conditions like chronic rhinosinusitis and certain cancers. Chronic rhinosinusitis involves excessive inflammation and tissue damage a process where CST1's inhibition of proteases like cathepsin B is important. In cancer abnormal CST1 expression levels have been noted in gastric and colorectal cancers suggesting a connection with tumor growth and metastasis. In these contexts altered expression or function of CST1 may interact with other proteins such as cathepsin L influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416), expandable thumbnail

    Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: HCT116 (PMID:24357805, PMID: 28300829, PMID: 19513549), 293T (PMID: 24357805).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution. Anti- Bovine Serum Albumin antibody (Anti-Albumin antibody [EPR12774] ab192603) loading control staining at 1/1000 dilution.
    Exposure time: 48 aeconds

    All lanes: Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416) at 1/1000 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

    Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 5: MDA-MB-231 culture supernatant at 10 µL

    Lane 6: SW480 culture supernatant at 10 µL

    Lane 7: HCT116 culture supernatant at 10 µL

    Lane 8: 293T culture supernatant at 10 µL

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 16 kDa

    Exposure time: 48s

  • Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416), expandable thumbnail

    Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human CST2 and CST4.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human CST1 expression vector containi a myc-his tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human CST2 expression vector containi a myc-his tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human CST4 expression vector containi a myc-his tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 16 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416), expandable thumbnail

    Immunoprecipitation - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416)

    Cystatin SN/CST1 was immunoprecipitated from 0.35 mg SW480 whole cell lysate with ab307416 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307416 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab307416 IP in SW480 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307416 in SW480 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-Cystatin SN/CST1 antibody [EPR25933-111] (ab307416) at 1/30 dilution

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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