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Rabbit Recombinant Monoclonal Cystatin SN/CST1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.

Alternative names

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Rabbit Recombinant Monoclonal Cystatin SN/CST1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25933-49

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab303701 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Cystatin SN/CST1.

The recommended pair for sandwich ELISA is:

Capture: ab303701, human Cystatin SN/CST1 Capture Antibody (unconjugated)

Detector: ab303702, human Cystatin SN/CST1 Detector Antibody (unconjugated)

The reference range value is 15.6-1000 pg/ml.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

Biological function summary

CST1 inhibits proteolytic enzymes that are involved in degrading proteins within cells and tissues. This protein is not part of a larger complex but instead exerts its function through one-on-one interactions with target proteases. Through its inhibitory actions CST1 plays a protective role in various bodily fluids and epithelial surfaces contributing to the body's barrier and immune defense mechanisms against pathogens and tissue damage.

Activity summary

Cystatin SN also known as CST1 is a cysteine protease inhibitor that belongs to the family of cystatins. This protein has a mass of approximately 16 kDa and is expressed primarily in human saliva as well as other tissues like the nasal mucosa and skin. Its main role involves binding to and inhibiting cathepsins and other cysteine protease enzymes preventing the breakdown of cellular and extracellular proteins. Such inhibition protects tissues from damage and helps maintain normal protein turnover.

Pathways

CST1 is engaged in the regulation of protease activity which impacts the broader proteolytic pathways that are important to cellular homeostasis. This regulation is linked to the lysosomal cathepsin family and pathways such as the protein metabolism pathway. Related proteins within these pathways include cathepsin L and cathepsin B which are also cysteine proteases that rely on precise regulation by cystatin family proteins like CST1 to prevent excessive protein degradation.

Associated diseases and disorders

CST1 has associations with conditions like chronic rhinosinusitis and certain cancers. Chronic rhinosinusitis involves excessive inflammation and tissue damage a process where CST1's inhibition of proteases like cathepsin B is important. In cancer abnormal CST1 expression levels have been noted in gastric and colorectal cancers suggesting a connection with tumor growth and metastasis. In these contexts altered expression or function of CST1 may interact with other proteins such as cathepsin L influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-Cystatin SN/CST1 antibody [EPR25933-49] - BSA and Azide free (Capture) (ab303701), expandable thumbnail

    Sandwich ELISA - Anti-Cystatin SN/CST1 antibody [EPR25933-49] - BSA and Azide free (Capture) (ab303701)

    Sandwich ELISA of ab303700 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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