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AB89606

Anti-Cysteine antibody [3A4]

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(1 Publication)

Mouse Monoclonal Cysteine antibody. Suitable for IP, ELISA, WB, IHC-Fr and reacts with Modified Amino Acid samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Cysteine.

View Alternative Names

(2R) 2 amino 3 sulfanyl propanoic acid

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A4

Isotype

IgG1

Carrier free

No

Applications

IP, ELISA, WB, IHC-Fr

applications

Specificity

Detects Cysteinylation of proteins from any source

Reactivity data

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.6 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 9% Urea, 0.395% Tris HCl, 0.0292% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cysteine often referred to in various forms such as cystine and cysteine is a sulfur-containing amino acid with a molecular weight of approximately 121.16 g/mol. In the mechanical aspect cysteine works as a building block of proteins by forming disulfide bonds that are important for the structural stability of proteins. These bonds particularly influence protein folding processes. Cysteine is expressed in many tissues and cells throughout the body with higher levels often observed in the liver skeletal muscles and skin.
Biological function summary

This amino acid functions beyond its role in protein structure by participating in enzymatic reactions and act as a precursor to the production of glutathione an important antioxidant that protects cells from oxidative stress. Cysteine can be found as a component of protein complexes involved in redox signaling. Through these complexes it supports cellular defense mechanisms and maintains a balance in the oxidative state of cells which is critical for proper cellular function.

Pathways

Cysteine's biological functions integrate into several essential pathways such as the methionine cycle and the glutathione synthesis pathway. Within the methionine cycle cysteine interacts with related proteins like homocysteine playing a role in methylation reactions critical for DNA and protein function. In the glutathione pathway cysteine directly contributes to the synthesis of glutathione interacting with enzymes like glutamate-cysteine ligase which enhances the cellular antioxidant capacity.

Cysteine is notably linked to liver disease and neurodegenerative disorders. Abnormal cysteine levels or disruptions in cysteine-related pathways can lead to oxidative stress which may exacerbate conditions like non-alcoholic fatty liver disease by affecting processes that involve interacting proteins such as S-adenosylmethionine. In neurodegenerative disorders like Alzheimer's disease cysteine-related dysregulation can impact proteins involved in oxidative stress responses potentially leading to neuronal damage and progression of the disease.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 12:e027769 PubMed36802924

2023

Role of PARP and TRPM2 in VEGF Inhibitor-Induced Vascular Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Karla B Neves,Rheure Alves-Lopes,Augusto C Montezano,Rhian M Touyz
View all publications

Product promise

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