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AB227003

Anti-CYTH4 antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal CYTH4 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYTH4.

View Alternative Names

CYT4, PSCD4, CYTH4, Cytohesin-4

1 Images
Western blot - Anti-CYTH4 antibody - N-terminal (AB227003)
  • WB

Supplier Data

Western blot - Anti-CYTH4 antibody - N-terminal (AB227003)

12% SDS PAGE gel.

All lanes:

Western blot - Anti-CYTH4 antibody - N-terminal (ab227003) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

CYTH4 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CYTH4. The exact immunogen used to generate this antibody is proprietary information.

Q9UIA0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein target CYTH4 also known as Cytohesin-4 functions mechanically as a guanine nucleotide exchange factor (GEF) for ADP-ribosylation factor (ARF). It facilitates the activation of ARF proteins by exchanging GDP for GTP critical for cellular actin dynamics and vesicular trafficking. CYTH4 is a cytosolic protein with a molecular mass of around 47 kDa and shows expression in various tissues including the brain placenta and liver. It contains Sec7 and pleckstrin homology (PH) domains that are key to its function and intracellular localization.
Biological function summary

CYTH4 regulates processes that involve actin cytoskeleton organization and membrane trafficking. It interacts with ARF proteins as part of a protein complex assisting in the coordination of endocytosis and exocytosis activities. Its role extends to cell adhesion and migration influencing cell shape and movement. This protein also participates in cell signaling pathways that govern cellular responses to external stimuli further highlighting its importance in maintaining cellular structure and communication.

Pathways

CYTH4 contributes significantly to the ARF signaling pathway and the integrin signaling pathway. Within the ARF pathway CYTH4 activates ARF proteins which are essential for both the trafficking of vesicles and actin remodelling. In the integrin pathway it modulates the interaction between the actin cytoskeleton and the cell membrane affecting cell adhesion and migration. CYTH4 has related interactions with other proteins such as integrins and small GTPases linking its activities to essential cellular processes.

CYTH4 has connections to cancer progression and neurological disorders. Its role in actin dynamics and cell movement makes it relevant in cancer metastasis where abnormal cell migration is a hallmark. Additionally CYTH4's regulation in neuronal cells suggests implications in neurodevelopmental disorders. Through these disease pathways CYTH4 associates with proteins like integrins which also play parts in modifying cell adhesion and migration properties.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP.
See full target information CYTH4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112634 PubMed40502711

2025

Cytohesin-4/ARF6 facilitates the progression of acute myeloid leukemia through activating PIK3R5/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fen Qiu,Cheng-Ming He,Yan-Mei Zeng,Xiao-Ling Deng,Guo-Lin Liang,Ming-Xing Zhong,Min Zou,Xiu-Juan Xiong,Jing-Dong Zhang,Yan Ye,Qing Niu,Xiao-Li Chen

Oncology letters 29:155 PubMed39911153

2025

Construction of a lung adenocarcinoma prognostic model based on KEAP1/NRF2/HO‑1 mutation‑mediated upregulated genes and bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhu,Ye Zhang,Lingyun Yang,Lu Chen,Chaobo Chen,Qifeng Shi,Zipeng Xu
View all publications
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Product promise

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