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AB248722

Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cytochrome b-c1 complex subunit 9/UQCR10 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

UCRC, HSPC119, UQCR10, Cytochrome b-c1 complex subunit 9, Complex III subunit 9, Complex III subunit X, Cytochrome c1 non-heme 7 kDa protein, Ubiquinol-cytochrome c reductase complex 7.2 kDa protein

2 Images
Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] - BSA and Azide free (AB248722)
  • WB

Unknown

Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] - BSA and Azide free (AB248722)

This data was developed using ab134909, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] (<a href='/en-us/products/primary-antibodies/cytochrome-b-c1-complex-subunit-9-uqcr10-antibody-epr8179-ab134909'>ab134909</a>) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Human fetal heart tissue lysate at 10 µg

Lane 5:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution

Predicted band size: 7 kDa

false

OI-RD Scanning - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] - BSA and Azide free (AB248722)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179] - BSA and Azide free (AB248722)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Unconjugated

    Anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody [EPR8179]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8179

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248722 is the carrier-free version of ab134909.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome b-c1 complex subunit 9 also known as UQCR10 is a small protein with a mass of approximately 7.6 kDa. It functions as a component of the cytochrome b-c1 complex which is an essential part of the mitochondrial electron transport chain. One can find UQCR10 expressed primarily in the inner mitochondrial membrane critical for cellular respiration and energy production. The role of UQCR10 within the complex is to stabilize the interaction between other subunits therefore maintaining the integrity of the cytochrome b-c1 complex.
Biological function summary

UQCR10 participates in facilitating electron transfer within the cytochrome b-c1 complex also known as Complex III of the mitochondrial respiratory chain. This complex comprises multiple protein subunits including cytochrome b cytochrome c1 and others. UQCR10 aids in the translocation of protons across the mitochondrial membrane contributing to the proton gradient necessary for ATP synthesis. This electron transfer process is vital for the production of cellular energy.

Pathways

The function of UQCR10 is integral to the oxidative phosphorylation pathway. This pathway involves several key processes within mitochondria that result in the synthesis of ATP. UQCR10 alongside cytochrome c1 and other subunits assists in the electron transfer from ubiquinol to cytochrome c. By facilitating these electron transfer reactions UQCR10 plays an important role in aerobic respiration and cellular energy metabolism.

UQCR10 has connections to mitochondrial disorders and neurodegenerative diseases. Mutations affecting the cytochrome b-c1 complex can lead to mitochondrial dysfunction impacting energy production and leading to conditions such as Leigh syndrome and other mitochondrial myopathies. The role of UQCR10 in stabilizing the cytochrome complex makes it relevant in understanding disease mechanisms alongside related proteins like cytochrome c1 that can influence disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.
See full target information UQCR10
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