JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236295

Anti-Cytochrome b245 Light Chain/p22-phox antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Cytochrome b245 Light Chain/p22-phox antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Cytochrome b-245 light chain aa 100 to C-terminus.

View Alternative Names

Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, CYBA

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)

Paraffin-embedded human lymph node tissue stained for Cytochrome b245 Light Chain/p22-phox using ab236295 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)

Paraffin-embedded human lung tissue stained for Cytochrome b245 Light Chain/p22-phox using ab236295 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB236295)

4% formaldehyde-fixed, 0.2% Triton X-100 permabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Cytochrome b245 Light Chain/p22-phox (green) using ab236295 at 1/66 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Cytochrome b-245 light chain aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13498

style
left-column

Alternative Products

Primary Antibodies

AB80896

Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1]

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody [44.1] (AB80896)

  • 0
  • 0
Primary Antibodies

AB191512

Anti-Cytochrome b245 Light Chain/p22-phox antibody

KO Validated

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB191512)

  • 0
  • 0
Primary Antibodies

AB75941

Anti-Cytochrome b245 Light Chain/p22-phox antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

  • 5
  • 5

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB179457

Anti-NCF1/p47-phox antibody [EPR13134]

RabMAb|Recombinant

Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (AB179457)

  • 1
  • 1
Primary Antibodies

AB223500

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327]

Recombinant|Lab Essentials|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cytochrome b245 Light Chain also known as p22-phox or CYBA plays a significant role in the NADPH oxidase complex. This protein has a molecular mass of approximately 22 kDa and is widely expressed in various cell types particularly in those associated with the immune system such as neutrophils. p22-phox forms part of the membrane-bound flavocytochrome b558 along with gp91-phox which is important for the generation of reactive oxygen species.
Biological function summary

The p22-phox serves as a core component of the NADPH oxidase complex which is integral to cellular responses to infection and stress. It operates in conjunction with other cytosolic subunits to produce reactive oxygen species that help in microbial destruction and signal transduction. The presence of p22-phox is essential for proper enzyme assembly and function as its interaction with gp91-phox establishes the electron transport chain required for oxidase activity.

Pathways

P22-phox contributes to the oxidative burst pathway a critical mechanism in innate immunity. In this pathway its interactions with proteins like Rac a small GTPase facilitate the activation and membrane association of the oxidase complex. Additionally p22-phox has involvement in inflammatory pathways by aiding in the release of signaling molecules that trigger inflammatory responses. This protein cooperates with components of the MAPK signaling pathway acting as a mediator in signal transduction events that dictate cellular responses to external stimuli.

P22-phox links closely with chronic granulomatous disease (CGD) a disorder characterized by a malfunctioning NADPH oxidase complex. Mutations in the CYBA gene can lead to defects in reactive oxygen species generation compromising the immune system's ability to combat infections. Furthermore evidence suggests a connection between p22-phox and cardiovascular diseases including hypertension where abnormalities in oxidase activity influence endothelial function. Proteins such as gp91-phox also share these disease associations highlighting the cooperative interaction of these components in disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 15824103, PubMed : 17140397, PubMed : 38355798). Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 19948736). Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide (PubMed : 15824103, PubMed : 17140397).
See full target information Cytochrome b-245 light chain
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed38346162

2024

A methylation-phosphorylation switch controls EZH2 stability and hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Guo,Rebecca C Lim,Keshari Rajawasam,Tiffany Trinh,Hong Sun,Hui Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com