AB75941

Anti-Cytochrome b245 Light Chain/p22-phox antibody

Name: Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) | Abcam
Brand: Abcam Limited
SKU: AB75941
Price: 510 USD
Availability: InStock
Rating: 5 (5 reviews)

BOND RX™ Validated
What is this?

(5 Reviews)

(32 Publications)

Rabbit Polyclonal Cytochrome b245 Light Chain/p22-phox antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 32 publications.

View Alternative Names

Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, Cyba

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

IHC image of Cytochrome b245 Light Chain/p22-phox staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab75941, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

Immunohistochemical analyses of p22phox using ab75941 (1/200) in normal adrenocortical tissue (b-c) and adrenal aldosterone-producing adenoma, (e-f) p22phox presents a gradient of expression in the entire adrenal cortex (100x), and its localization was restricted to the cell membrane (400x). Sections were incubated with primary antibody at 4°C overnight and with a HRP-conjugated secondary antibody for 30 minutes at 37°C.

Geng X et al., Int J Endocrinol 2016, Fig 3., doi: 10.1155/2016/2395634 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Unknown

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

We hypothesize that the 21 kDa band represents the pro-form of Cytochrome b245 Light Chain/p22-phox, and the 19 kDa band represents the mature form.

All lanes:

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (ab75941) at 1 µg/mL

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Pancreas (Mouse) Tissue Lysate at 10 µg

Lane 3:

Spleen (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa,21 kDa,40 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of ab75941 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee. Please note, previous lots of this product were also guaranteed in ICC/IF. We are having trouble reproducing new lots suitable for ICC/IF. Therefore this application has been removed and it no longer guaranteed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cytochrome b245 Light Chain also known as p22-phox or CYBA plays a significant role in the NADPH oxidase complex. This protein has a molecular mass of approximately 22 kDa and is widely expressed in various cell types particularly in those associated with the immune system such as neutrophils. p22-phox forms part of the membrane-bound flavocytochrome b558 along with gp91-phox which is important for the generation of reactive oxygen species.

Biological function summary

The p22-phox serves as a core component of the NADPH oxidase complex which is integral to cellular responses to infection and stress. It operates in conjunction with other cytosolic subunits to produce reactive oxygen species that help in microbial destruction and signal transduction. The presence of p22-phox is essential for proper enzyme assembly and function as its interaction with gp91-phox establishes the electron transport chain required for oxidase activity.

Pathways

P22-phox contributes to the oxidative burst pathway a critical mechanism in innate immunity. In this pathway its interactions with proteins like Rac a small GTPase facilitate the activation and membrane association of the oxidase complex. Additionally p22-phox has involvement in inflammatory pathways by aiding in the release of signaling molecules that trigger inflammatory responses. This protein cooperates with components of the MAPK signaling pathway acting as a mediator in signal transduction events that dictate cellular responses to external stimuli.

P22-phox links closely with chronic granulomatous disease (CGD) a disorder characterized by a malfunctioning NADPH oxidase complex. Mutations in the CYBA gene can lead to defects in reactive oxygen species generation compromising the immune system's ability to combat infections. Furthermore evidence suggests a connection between p22-phox and cardiovascular diseases including hypertension where abnormalities in oxidase activity influence endothelial function. Proteins such as gp91-phox also share these disease associations highlighting the cooperative interaction of these components in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor (By similarity). Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide (By similarity).

See full target information Cytochrome b-245 light chain

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang

PloS one 20:e0318008 PubMed40179080

2025

Allopurinol attenuates development of Porphyromonas gingivalis LPS-induced cardiomyopathy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Akinaka Morii,Ichiro Matsuo,Kenji Suita,Yoshiki Ohnuki,Misao Ishikawa,Aiko Ito,Go Miyamoto,Mariko Abe,Takao Mitsubayashi,Yasumasa Mototani,Megumi Nariyama,Ren Matsubara,Yoshio Hayakawa,Yasuharu Amitani,Kazuhiro Gomi,Takatoshi Nagano,Satoshi Okumura

Aging 16:11062-11071 PubMed38975935

2024

Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

An Xie,Xianjie Zhang,Feng Ju,Yukai Zhou,Dan Wu,Jia Han

iScience 27:108868 PubMed38318360

2024

Nox4-SH3YL1 complex is involved in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sae Rom Lee,Hye Eun Lee,Jung-Yeon Yoo,Eun Jung An,Soo-Jin Song,Ki-Hwan Han,Dae Ryong Cha,Yun Soo Bae

Aging 15:12264-12274 PubMed37934569

2023

Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan Lv

Infection and immunity 91:e0025823 PubMed37874162

2023

Detoxification of reactive oxygen species by the hyaluronic acid capsule of group A .

Applications

Unspecified application

Species

Unspecified reactive species

Shyra Wilde,Ananya Dash,Anders Johnson,Kialani Mackey,Cheryl Y M Okumura,Christopher N LaRock

International journal of molecular sciences 24: PubMed37762672

2023

Stachydrine Hydrochloride Regulates the NOX2-ROS-Signaling Axis in Pressure-Overload-Induced Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Lu,Yueyang Liang,Songru Yang,Mengwei Fu,Xiaoli Shan,Chen Zhang,Huihua Chen,Pei Zhao,Rong Lu

Biomolecules 13: PubMed37189375

2023

Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Toma,Gabriela M Sanda,Camelia S Stancu,Loredan S Niculescu,Mina Raileanu,Anca V Sima

Antioxidants (Basel, Switzerland) 12: PubMed36830003

2023

Targeting M2 Macrophages with a Novel NADPH Oxidase Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Sébastien Dilly,Miguel Romero,Stéphanie Solier,Olivier Feron,Chantal Dessy,Anny Slama Schwok

Molecular biology reports 50:2067-2076 PubMed36542238

2022

Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh Kumar,Shubhankar Suman,Bo-Hyun Moon,Albert J Fornace,Kamal Datta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome b245 Light Chain/p22-phox antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

Western blot - Anti-Cytochrome b245 Light Chain/p22-phox antibody (AB75941)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Product promise