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AB316356

Anti-Cytochrome b245 Light Chain/p22-phox antibody [CS9]

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Mouse Monoclonal Cytochrome b245 Light Chain/p22-phox antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human Cytochrome b-245 light chain.

View Alternative Names

Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, CYBA

5 Images
Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Blue line : Flow cytometric analysis of MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Unknown Specificity Isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Blue line : Flow cytometric analysis of MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Unknown Specificity Isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CS9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human Cytochrome b-245 light chain.

P13498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cytochrome b245 Light Chain also known as p22-phox or CYBA plays a significant role in the NADPH oxidase complex. This protein has a molecular mass of approximately 22 kDa and is widely expressed in various cell types particularly in those associated with the immune system such as neutrophils. p22-phox forms part of the membrane-bound flavocytochrome b558 along with gp91-phox which is important for the generation of reactive oxygen species.
Biological function summary

The p22-phox serves as a core component of the NADPH oxidase complex which is integral to cellular responses to infection and stress. It operates in conjunction with other cytosolic subunits to produce reactive oxygen species that help in microbial destruction and signal transduction. The presence of p22-phox is essential for proper enzyme assembly and function as its interaction with gp91-phox establishes the electron transport chain required for oxidase activity.

Pathways

P22-phox contributes to the oxidative burst pathway a critical mechanism in innate immunity. In this pathway its interactions with proteins like Rac a small GTPase facilitate the activation and membrane association of the oxidase complex. Additionally p22-phox has involvement in inflammatory pathways by aiding in the release of signaling molecules that trigger inflammatory responses. This protein cooperates with components of the MAPK signaling pathway acting as a mediator in signal transduction events that dictate cellular responses to external stimuli.

P22-phox links closely with chronic granulomatous disease (CGD) a disorder characterized by a malfunctioning NADPH oxidase complex. Mutations in the CYBA gene can lead to defects in reactive oxygen species generation compromising the immune system's ability to combat infections. Furthermore evidence suggests a connection between p22-phox and cardiovascular diseases including hypertension where abnormalities in oxidase activity influence endothelial function. Proteins such as gp91-phox also share these disease associations highlighting the cooperative interaction of these components in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
See full target information Cytochrome b-245 light chain
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Product promise

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