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AB228751

Anti-Cytochrome b5 antibody

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(1 Publication)

Rabbit Polyclonal Cytochrome b5 antibody. Suitable for WB, ICC/IF and reacts with Human, Dog samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYB5A.

View Alternative Names

CYB5, CYB5A, Cytochrome b5, Microsomal cytochrome b5 type A, MCB5

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Immunofluorescence analysis of PG-4 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Cytochrome b5 protein at cytoplasm using ab228751 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b5 antibody (AB228751)

Immunofluorescence analysis of MDCK cells fixed in 4% paraformaldehyde at RT for 15 min labelling Cytochrome b5 protein at cytoplasm using ab228751 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-Cytochrome b5 antibody (AB228751)
  • WB

Supplier Data

Western blot - Anti-Cytochrome b5 antibody (AB228751)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cytochrome b5 antibody (ab228751) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Cytochrome b5-transfected HEK-293T cell lysate

Predicted band size: 15 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Dog, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CYB5A. The exact immunogen used to generate this antibody is proprietary information.

P00167

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome b5 also known as CYB5A is a small hemoprotein with a molecular mass of approximately 16.7 kDa. It plays a role as electron carrier for various oxidative reactions involving oxygenases and reductases. This protein exists in two soluble forms: a microsomal form found in the endoplasmic reticulum and a mitochondrial form located in the outer mitochondrial membrane. Cytochrome b5 is expressed in many tissues including liver kidney and skin where it assists in metabolic processes.
Biological function summary

The functions of Cytochrome b5 include participation in fatty acid desaturation and elongation as well as cholesterol and steroid synthesis. It interacts with various enzyme systems including the cytochrome P450 enzyme complex acting as an electron donor in these biosynthetic pathways. Cytochrome b5 contributes to the regulation of these processes ensuring proper lipid composition in cellular membranes.

Pathways

Cytochrome b5 contributes to the electron transport chain and xenobiotic metabolism pathways. In the electron transport chain it collaborates with Cytochrome P450 to facilitate monooxygenase reactions essential for metabolizing drugs and detoxification processes. Additionally Cytochrome b5 affects fatty acid metabolism by introducing double bonds into fatty acid chains interacting with stearoyl-CoA desaturase.

Cytochrome b5 plays a role in hereditary methemoglobinemia and some cases of androgen insensitivity syndrome. Mutations affecting Cytochrome b5 can disrupt electron transfer leading to elevated levels of methemoglobin and compromised oxygen delivery. In androgen insensitivity syndrome defects in the electron transfer process with other proteins like Cytochrome P450 can affect steroid metabolism and hormonal balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
See full target information CYB5A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 9:596154 PubMed33634081

2021

and Are Putative Modulators of Corneoscleral Stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Boazak,Rebecca King,Jiaxing Wang,Cassandra M Chu,Aaron M Toporek,Joseph M Sherwood,Darryl R Overby,Eldon E Geisert,C Ross Ethier
View all publications
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Product promise

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