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Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody that is used to detect Cytochrome C in Western Blot, Flow Cytometry, ICC/IF. Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.



- Over 130 publications


Images

Western blot - Anti-Cytochrome C antibody [37BA11] (AB110325), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325), expandable thumbnail
  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (AB110325), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (AB110325), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Caenorhabditis elegans
Predicted
Expected
Predicted
Cow
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Cow, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Caenorhabditis elegans
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
0.25-1 µg/mL
Notes

-

Species
Cow
Dilution info
0.25-1 µg/mL
Notes

-

Species
Mouse
Dilution info
0.25-1 µg/mL
Notes

-

Species
Rat
Dilution info
0.25-1 µg/mL
Notes

-

Expected
Expected

Species
Caenorhabditis elegans
Dilution info
0.25-1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
0.5-2 µg/mL
Notes

Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.

Expected
Expected

Species
Cow, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Caenorhabditis elegans
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Alternative names

CYC, CYCS, Cytochrome c

Recommended products

Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody that is used to detect Cytochrome C in Western Blot, Flow Cytometry, ICC/IF. Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.



- Over 130 publications

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
37BA11
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...
Purification notes

ab110325 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

What is this antibody validated in?


Anti-Cytochrome C antibody [37BA11] (ab110325) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.

What is the molecular weight of Cytochrome C?


Anti-Cytochrome C [37BA11] (ab110325) specifically detects a band for Cytochrome C (UniProt: P99999) at a molecular weight of 12kDa.

Recommended positive controls


Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells.

Trusted by the scientific community


Anti-Cytochrome C [37BA11] (ab110325) was first used in a scientific publication in 2011 and has been cited over 130 times in peer-reviewed journals.

Other related products


We have a range of other formats of antibody clone [37BA11] also available for your convenience:
ab110325, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] ab154476, HRP - HRP Anti-Cytochrome C antibody [37BA11] ab198489, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] ab198583



Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Associated diseases and disorders

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

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5 product images

  • Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325), expandable thumbnail

    Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325)

    All lanes: Western blot - Anti-Cytochrome C antibody [37BA11] (ab110325) at 1 µg/mL

    Lane 1: Isolated mitochondria from Human heart at 5 µg

    Lane 2: Isolated mitochondria from Bovine heart at 1 µg

    Lane 3: Isolated mitochondria from Rat heart at 10 µg

    Lane 4: Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 11 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)

    ab110325 staining Cytochrome C in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted DyLight® 550-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

  • Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325), expandable thumbnail

    Flow Cytometry - Anti-Cytochrome C antibody [37BA11] (ab110325)

    Flow cytometric analysis using ab110325 at 1µg/ml staining Cytochrome C in HeLa cells (blue). Isotype control antibody (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)

    ab110325 staining cytochrome C in MCF7 cells treated with 15-Deoxy-delta12,14-prostaglandin J2 (15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist ab141717), by ICC/IF. Expression of cytochrome C changes from mitochondrial puncta to a difuse staining pattern with increased concentration of 15-Deoxy-delta12,14-prostaglandin J2, as described in literature.
    The cells were incubated at 37°C for 24 hours in media containing different concentrations of 15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist ab141717 (15-Deoxy-delta12,14-prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab110325 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [37BA11] (ab110325)

    Immunocytochemistry analysis using ab110325 at 2μg/ml staining Cytochrome C in Human fibroblasts (fixed and permeabilized), followed by a fluorescent goat-anti-mouse IgG.

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Product protocols

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