Skip to main content

Mouse Monoclonal Cytochrome C antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Full Length Protein corresponding to Rat Cycs.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (AB270249), expandable thumbnail
  • Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (AB270249), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Rat Cycs. The exact immunogen used to generate this antibody is proprietary information. Database link P62898

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Alternative names

CYC, CYCS, Cytochrome c

Recommended products

Mouse Monoclonal Cytochrome C antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Full Length Protein corresponding to Rat Cycs.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Rat Cycs. The exact immunogen used to generate this antibody is proprietary information. Database link P62898
Clone number
6H2.B4
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Associated diseases and disorders

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (ab270249), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [6H2.B4] (ab270249)

    Immunofluorescence analysis of HeLa cells labeling Cytochrome C (Green) using ab270249 at 2μg/ml, followed by goat anti-mouse IgG-CF488 (Green).

  • Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (ab270249), expandable thumbnail

    Flow Cytometry - Anti-Cytochrome C antibody [6H2.B4] (ab270249)

    Flow cytometric analysis of PFA-fixed HeLa cells labeling cytochrome C using ab270249 at 2μg/106 cells followed by goat anti-mouse IgG-CF488 (Blue); goat anti-mouse IgG-CF488 is control (Red).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com