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Mouse Monoclonal Cytochrome C antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 208 publications.
Alternative names=Cytochrome c, CYCS, CYC
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted | Predicted |
| Pigeon | Predicted | Predicted | Predicted | Predicted |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Pigeon, Drosophila melanogaster | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-1 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Pigeon, Drosophila melanogaster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Pigeon, Drosophila melanogaster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Pigeon, Drosophila melanogaster | Dilution info - | Notes - |
Target data
Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Recommended products
Mouse Monoclonal Cytochrome C antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 208 publications.
Alternative names=Cytochrome c, CYCS, CYC
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Clone number
7H8.2C12
- Purification technique
Affinity purification Protein G
- Epitope
The antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, based on competitive ELISA results.
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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