AB18738

Anti-Cytochrome C antibody

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(14 Publications)

Rabbit Polyclonal Cytochrome C antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Rat Cycs.

View Alternative Names

CYC, CYCS, Cytochrome c

3 Images

Supplier Data

Western blot - Anti-Cytochrome C antibody (AB18738)

Western Blot analysis of Left Lane : Jurkat cell lysate and Right Lane : 3T3 cell lysates labeling Cytochrome C with ab18738.

All lanes:

Western blot - Anti-Cytochrome C antibody (ab18738)

Predicted band size: 11 kDa

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Supplier Data

Western blot - Anti-Cytochrome C antibody (AB18738)

All lanes:

Western blot - Anti-Cytochrome C antibody (ab18738)

All lanes:

Rat kidney cell lysates

Predicted band size: 11 kDa

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CiteAb

Western blot - Anti-Cytochrome C antibody (AB18738)

Cytochrome C western blot using anti-Cytochrome C antibody ab18738. Publication image and figure legend from Liu, H., Zhao, J., et al., 2019, PLoS One, PubMed 31091245.

ab18738 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab18738 please see the product overview.

Rk3 induces apoptosis in Eca109 and KYSE150 cells.(A) Hoechst 33342 staining. Scale bar : 100 μm. (B) Ultrastructure of apoptotic cells was observed by TEM. Scale bar : 1 μm. (C) Apoptosis was quantified by flow cytometry. (D) Effect of different doses of Rk3 on the expression of apoptosis-related proteins after treatment for 24 h. (E) Immunohistochemistry staining of tumor tissue. *p < 0.05, **p < 0.01 compared with control.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Rat Cycs. The exact immunogen used to generate this antibody is proprietary information.

P62898

Specificity

This antibody detects the 15 kDa cytochrome C.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3025 Cytochrome c Antibody. 3025-100 is the same size as the 100 µg size of ab18738.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.03% Proclin 300 Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information CYCS

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:33520 PubMed41023048

2025

SETD5 in glioma cells conferred TRAIL resistance induction.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshay Taneja,Sachin Bhardwaj,Ajay Kumar Yadav

Translational andrology and urology 10:2051-2066 PubMed34159086

2021

The protective effects of exogenous spermine on renal ischemia-reperfusion injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yan,Shao-Ju Min,Bin Xu,Cheng Zhang,Jun Pei,Wei Zhang,Guang-Heng Luo

FASEB journal : official publication of the Federa 33:13503-13514 PubMed31570002

2019

Serine-47 phosphorylation of cytochrome in the mammalian brain regulates cytochrome oxidase and caspase-3 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hasini A Kalpage,Asmita Vaishnav,Jenney Liu,Ashwathy Varughese,Junmei Wan,Alice A Turner,Qinqin Ji,Matthew P Zurek,Alexandr A Kapralov,Valerian E Kagan,Joseph S Brunzelle,Maurice-Andre Recanati,Lawrence I Grossman,Thomas H Sanderson,Icksoo Lee,Arthur R Salomon,Brian F P Edwards,Maik Hüttemann

PloS one 14:e0216759 PubMed31091245

2019

The ginsenoside Rk3 exerts anti-esophageal cancer activity in vitro and in vivo by mediating apoptosis and autophagy through regulation of the PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Liu,Jiaqi Zhao,Rongzhan Fu,Chenhui Zhu,Daidi Fan

Journal of cellular physiology 234:22439-22449 PubMed31087336

2019

Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Li,Weihong Zhang,Hong Zhang,Yi Xie,Chao Sun,Cuixia Di,Jing Si,Lu Gan,Junfang Yan

Experimental and therapeutic medicine 17:2152-2160 PubMed30783480

2019

MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Lusha E,Hong Jiang,Zhibing Lu

Comparative biochemistry and physiology. Part B, B 219-220:1-9 PubMed29501789

2018

A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection.

Applications

Species

Unspecified reactive species

Ana Stancic,Aleksandra Jankovic,Aleksandra Korac,Dusko Cirovic,Vesna Otasevic,Kenneth B Storey,Bato Korac

Cell 171:1125-1137.e11 PubMed29107333

2017

Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program.

Applications

Unspecified application

Species

Unspecified reactive species

Farokh Dotiwala,Sumit Sen Santara,Andres Ariel Binker-Cosen,Bo Li,Sriram Chandrasekaran,Judy Lieberman

PLoS genetics 13:e1006744 PubMed28426667

2017

Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

Applications

Species

Unspecified reactive species

Penelope J Boyd,Wen-Yo Tu,Hannah K Shorrock,Ewout J N Groen,Roderick N Carter,Rachael A Powis,Sophie R Thomson,Derek Thomson,Laura C Graham,Anna A L Motyl,Thomas M Wishart,J Robin Highley,Nicholas M Morton,Thomas Becker,Catherina G Becker,Paul R Heath,Thomas H Gillingwater

Biology of reproduction 91:68 PubMed25078683

2014

The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries.

Applications

Unspecified application

Species

Mouse

Sudipta Dutta,Connie J Mark-Kappeler,Patricia B Hoyer,Melissa E Pepling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome C antibody

Western blot - Anti-Cytochrome C antibody (AB18738)

Western blot - Anti-Cytochrome C antibody (AB18738)

Western blot - Anti-Cytochrome C antibody (AB18738)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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