AB76237

Anti-Cytochrome C antibody [EP1326-80-5-4]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(18 Publications)

Rabbit Recombinant Monoclonal Cytochrome C antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

View Alternative Names

CYC, CYCS, Cytochrome c

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

ab76237 at a 1/100 dilution staining human Cytochrome C in heart muscle by Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

ab76237 at a 1/100 dilution staining human Cytochrome C in kidney by Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

All lanes:

Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] (ab76237) at 1/500 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 14 kDa

false

Carrier free

Anti-Cytochrome C antibody [EP1326-80-5-4] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1326-80-5-4

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information CYCS

style

left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:30848-30862 PubMed39463189

2024

Transdermal Minimally Invasive Optical Multiplex Detection of Protein Biomarkers by Nanopillars Array-Embedded Microneedles.

Applications

Unspecified application

Species

Unspecified reactive species

Adva Raz,Hila Gubi,Adam Cohen,Fernando Patolsky

iScience 27:109468 PubMed38550985

2024

Creatine and low-dose lithium supplementation separately alter energy expenditure, body mass, and adipose metabolism for the promotion of thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

M S Finch,G L Gardner,J L Braun,M S Geromella,J Murphy,K Colonna,R Dhaliwal,A Retta,A Mohammad,J A Stuart,P J LeBlanc,V A Fajardo,B D Roy,R E K MacPherson

The Journal of biological chemistry 298:102568 PubMed36209826

2022

Low-dose lithium supplementation promotes adipose tissue browning and sarco(endo)plasmic reticulum Ca ATPase uncoupling in muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mia S Geromella,Chantal R Ryan,Jessica L Braun,Michael S Finch,Lucas A Maddalena,Olivia Bagshaw,Briana L Hockey,Fereshteh Moradi,Rachel K Fenech,Jisook Ryoo,Daniel M Marko,Roopan Dhaliwal,Jake Sweezey-Munroe,Sophie I Hamstra,Georgina Gardner,Sebastian Silvera,Rene Vandenboom,Brian D Roy,Jeffrey A Stuart,Rebecca E K MacPherson,Val A Fajardo

Physiological reports 10:e15232 PubMed35312183

2022

Subcutaneous adipose tissue sclerostin is reduced and Wnt signaling is enhanced following 4-weeks of sprint interval training in young men with obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Nigel Kurgan,Hashim Islam,Jennifer B L Matusiak,Bradley J Baranowski,Joshua Stoikos,Val A Fajardo,Rebecca E K MacPherson,Brendon J Gurd,Panagiota Klentrou

Aging 13:22502-22515 PubMed34580235

2021

Melatonin inhibits proliferation, migration, and invasion by inducing ROS-mediated apoptosis via suppression of the PI3K/Akt/mTOR signaling pathway in gallbladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kunlun Chen,Pengfei Zhu,Wenhui Chen,Kai Luo,Xiao-Jing Shi,Wenlong Zhai

Experimental and therapeutic medicine 22:1234 PubMed34539830

2021

Geniposide protects pulmonary arterial smooth muscle cells from lipopolysaccharide-induced injury via α7nAchR-mediated TLR-4/MyD88 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

San-Ying Shen,Li-Quan Ren,Hui-Dong Chen,Hong-Fei Zhu,Deng-Feng Zhou,Bo Zhang,Xiao-Qin Tan,Yong-Hua Xie

Nutrients 13: PubMed34371916

2021

Creatine Monohydrate Supplementation Increases White Adipose Tissue Mitochondrial Markers in Male and Female Rats in a Depot Specific Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Chantal R Ryan,Michael S Finch,Tyler C Dunham,Jensen E Murphy,Brian D Roy,Rebecca E K MacPherson

Biochemistry research international 2020:5253108 PubMed33489376

2020

Interaction of Agaric Acid with the Adenine Nucleotide Translocase Induces Mitochondrial Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Edmundo Chávez,Mabel Buelna-Chontal,Arturo Macías-López,Luz Hernández-Esquivel,Francisco Correa,Natalia Pavón

Redox biology 25:101109 PubMed30686776

2019

HIF-1α protects against oxidative stress by directly targeting mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Sheng Li,Yan-Ni Zhou,Lu Li,Sheng-Fu Li,Dan Long,Xue-Lu Chen,Jia-Bi Zhang,Li Feng,You-Ping Li

Cellular physiology and biochemistry : internation 51:1982-1995 PubMed30513524

2018

Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kaneko,Julian P Tuazon,Xunming Ji,Cesario V Borlongan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome C antibody [EP1326-80-5-4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

Western blot - Anti-Cytochrome C antibody [EP1326-80-5-4] (AB76237)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise