Rabbit Recombinant Monoclonal Cytochrome C antibody. Suitable for mIHC, IHC-P, WB and reacts with Human, Mouse, African green monkey samples. Cited in 8 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137205.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)")
![Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--multiplex-immunohistochemistry-img410093.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)")
![Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--multiplex-immunohistochemistry-img410084.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)")
Publications
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- International journal of biological sciences 19:5233-52442023Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaixia Li,Dong Chen,Xiaoqin Zhang,Mingxian Chen,Yinghao Zhi,Weilu Cui,Shanshan Li,Fan Xu,Ying Tan,Hao Zhou,Xing Chang,Hengwen ChenPubMed 37928261
- Bioengineered 13:4100-41112022Angiotensin-(1-7) ameliorates high glucose-induced vascular endothelial injury through suppressing chloride channel 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Cheng,Jing Liu,Zhuolin Guo,Shicheng Li,Jingfu Chen,Chang Tu,Fengzhou Fu,Bai Shen,Xiaojie Zhang,Guohua Lai,Jun LanPubMed 35098884
- Molecular medicine reports 25:2022Epigallocatechin gallate enhances human lens epithelial cell survival after UVB irradiation via the mitochondrial signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuxin Wu,Jike Song,Yan'e Gao,Yingying Zou,Junguo Guo,Xiuyan Zhang,Dongmei Liu,Dadong Guo,Hongsheng BiPubMed 35039875
- Nature communications 11:30672020Hepatic HuR modulates lipid homeostasis in response to high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuojun Zhang et. al.PubMed 32546794
- Cell death & disease 10:9592019TIPRL potentiates survival of lung cancer by inducing autophagy through the eIF2α-ATF4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu-Jin Jeon et. al.PubMed 31862913
- Lasers in surgery and medicine 52:424-4362019Effects of Pulsed 810 nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaurav K Keshri,Anju Yadav,Saurabh Verma,Bhuvnesh Kumar,Asheesh GuptaPubMed 31483061
- Phytomedicine : international journal of phytother 36:95-107201716-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Liu et. al.PubMed 29157834
- Biomedicine & pharmacotherapy = Biomedecine & phar 91:664-6722017The protective effect of propofol against TNF-α-induced apoptosis was mediated via inhibiting iNOS/NO production and maintaining intracellular Ca homeostasis in mouse hippocampal HT22 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Xu et. al.PubMed 28499237
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| mIHC | IHC-P | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Expected | Tested | Not recommended | Tested |
| Rat | Predicted | Predicted | Not recommended | Predicted |
| African green monkey | Expected | Expected | Not recommended | Tested |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, African green monkey, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species African green monkey | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Alternative names
CYC, CYCS, Cytochrome c
Recommended products
Rabbit Recombinant Monoclonal Cytochrome C antibody. Suitable for mIHC, IHC-P, WB and reacts with Human, Mouse, African green monkey samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1326-80-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
- Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
- Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
- Associated diseases and disorders
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--western-blot-img138096.gif "Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)")
Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
All lanes: Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: COS cell lysate at 10 µg
Lane 3: L929 cell lysate at 10 µg
SecondaryLanes 1 - 2: Goat anti-rabbit HRP at 1/2000 dilution
Lane 3: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 15 kDa
![Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--multiplex-immunohistochemistry-img410093.jpg "Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)")
Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
Fluorescence multiplex immunohistochemical analysis of the Human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-CaSR (Anti-CaSR antibody [EPR24050-59] ab259846, magenta; Opal™690), anti-Cytochrome C (Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, green; Opal™520) and anti-FABP4 (Anti-FABP4 antibody [EPR3579] ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-CaSR stained on parathyroid chief cells. Panel C: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-CaSR antibody [EPR24050-59] ab259846 at 1/5000 dilution (0.103 μg/ml), Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438 at 1/5000 dilution (0.195 μg/ml), and Anti-FABP4 antibody [EPR3579] ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This data was developed using Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, the same antibody clone in a different buffer formulation.![Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-ep1326-80-5-ab76107--multiplex-immunohistochemistry-img410084.jpg "Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)")
Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107)
Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Parathyroid Hormone (Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free ab236229, magenta; Opal™690), anti-Cytochrome C (Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, green; Opal™520) and anti-FABP4 (Anti-FABP4 antibody [EPR3579] ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C: anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438 at 1/5000 dilution (0.195 μg/ml) and Anti-FABP4 antibody [EPR3579] ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This data was developed using Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, the same antibody clone in a different buffer formulation.
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