Anti-Cytochrome C antibody [EP1326-80-5]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)

ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)

Fluorescence multiplex immunohistochemical analysis of the Human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-CaSR (ab259846, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B : anti-CaSR stained on parathyroid chief cells. Panel C : anti-Cytochrome C stained on parathyroid oxyphil cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab259846 at 1/5000 dilution (0.103 μg/ml), ab247438 at 1/5000 dilution (0.195 μg/ml), and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This data was developed using ab247438, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)

Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B : anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C : anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then ab247438 at 1/5000 dilution (0.195 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This data was developed using ab247438, the same antibody clone in a different buffer formulation.

• WB

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Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (AB76107)

All lanes:

Western blot - Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

COS cell lysate at 10 µg

Lane 3:

L929 cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Goat anti-rabbit HRP at 1/2000 dilution

Lane 3:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 11 kDa

Observed band size: 15 kDa

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