Name: Anti-Cytochrome C antibody [EPR1327]
SKU: ab133504
Rating: 5 (2 reviews)

Anti-Cytochrome C antibody [EPR1327] (ab133504) is a rabbit monoclonal antibody that is used to detect Cytochrome C in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 230 publications

Related conjugates and formulations

ab192485
Conjugated
Alexa Fluor® 488 Anti-Cytochrome C antibody [EPR1327]
ab192486
Conjugated
Alexa Fluor® 647 Anti-Cytochrome C antibody [EPR1327]
ab218312
Carrier free
Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free
ab193239
Conjugated
HRP Anti-Cytochrome C antibody [EPR1327]
ab317900
Conjugated
PE Anti-Cytochrome C antibody [EPR1327]
ab317940
Conjugated
APC Anti-Cytochrome C antibody [EPR1327]
ab318064
Conjugated
Alexa Fluor® 555 Anti-Cytochrome C antibody [EPR1327]
ab318022
Conjugated
Alexa Fluor® 594 Anti-Cytochrome C antibody [EPR1327]
ab321049
Conjugated
Alexa Fluor® 750 Anti-Cytochrome C antibody [EPR1327]

Images

Western blot - Anti-Cytochrome C antibody [EPR1327] (AB133504), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected
Rat	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -
Species Human	Dilution info 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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11 products for Alternative Product

Target data

See full target information CYCS

Function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Alternative names

CYC, CYCS, Cytochrome c

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR1327
Purification technique: Affinity purification Protein A
Dissociation constant: 1.29 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-Cytochrome C antibody [EPR1327] (ab133504) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Cytochrome C?

Anti-Cytochrome C [EPR1327] (ab133504) specifically detects a band for Cytochrome C (UniProt: P99999) at a molecular weight of 11kDa.

Trusted by the scientific community

Anti-Cytochrome C [EPR1327] (ab133504) was first used in a scientific publication in 2012 and has been cited over 230 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR1327] also available for your convenience:
ab133504, Alexa Fluor® 488 - ab192485, Alexa Fluor® 647 - ab192486, HRP - ab193239, Carrier free - ab218312, PE - ab317900, APC - ab317940, Alexa Fluor® 594 - ab318022, Alexa Fluor® 555 - ab318064, Alexa Fluor® 750 - ab321049

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Associated diseases and disorders

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution
All lanes: Human fetal kidney tissue lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunofluorescence staining of SH-SY5Y cells with purified ab133504 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1 in 500. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified ab133504 was used at a dilution of 1/200 followed by an Alexa Fluor^® 594 goat anti-mouse antibody at a dilution of 1/500.
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling Cytochrome C with unpurified ab133504 at 1/100 dilution.
Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution
All lanes: Human fetal heart tissue lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/5000 dilution
All lanes: Rat brain tissue lysate at 1/1000 dilution
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/50000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] (ab133504)
ab133504 (purified) at 1/30 immunoprecipitating Cytochrome C in Molt-4 cells.
For western blotting, a HRP-conjugated goat anti-rabbit (H+L), was used as the secondary antibody (1/1000).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunohistochemical staining of paraffin embedded mouse liver with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunohistochemical staining of paraffin embedded rat kidney with purified ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] (ab133504)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504)
All lanes: Western blot - Anti-Cytochrome C antibody [EPR1327] (ab133504) at 1/10000 dilution
Lane 1: Molt4 lysate at 10 µg
Lane 2: SH-SY5Y lysate at 10 µg
Lane 3: Human heart lysate at 10 µg
Lane 4: Human kidney lysate at 10 µg
Lane 5: Human spleen lysate at 10 µg
Secondary
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] (ab133504)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Cytochrome C with unpurified ab133504 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.