Rabbit Recombinant Monoclonal Cytochrome C antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunocytochemistry-immunofluorescence-img106530.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312)")
![Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunoprecipitation-img43804.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19230.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (AB218312)")
Publications
Filter by
- Redox biology 10:233-2422016Hydroxytyrosol inhibits hydrogen peroxide-induced apoptotic signaling via labile iron chelation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Kitsati et. al.PubMed 27810738
- PloS one 11:e01609822016Ethanol and Acetaminophen Synergistically Induce Hepatic Aggregation and TCH346-Insensitive Nuclear Translocation of GAPDH.
- Free radical biology & medicine 97:454-4652016Intracellular labile iron determines H2O2-induced apoptotic signaling via sustained activation of ASK1/JNK-p38 axis.
- Scientific reports 6:213822016Mark4 promotes oxidative stress and inflammation via binding to PPARγ and activating NF-κB pathway in mice adipocytes.
- Frontiers in aging neuroscience 7:2042015PGC-1α Silencing Compounds the Perturbation of Mitochondrial Function Caused by Mutant SOD1 in Skeletal Muscle of ALS Mouse Model.
- International journal of molecular medicine 36:1487-962015Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro.
- Cell death & disease 5:e13732014The protease Omi regulates mitochondrial biogenesis through the GSK3β/PGC-1α pathway.
- Journal of immunology (Baltimore, Md. : 1950) 191:2614-232013Enhancer of zeste homolog 2 is a negative regulator of mitochondria-mediated innate immune responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuai Chen et. al.PubMed 23918984
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
3 products for Alternative Version
Target data
Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Alternative names
CYC, CYCS, Cytochrome c
Recommended products
Rabbit Recombinant Monoclonal Cytochrome C antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1327
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.29 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab218312 is the carrier-free version of Anti-Cytochrome C antibody [EPR1327] ab133504.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.
- Biological function summary
Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.
- Pathways
Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.
- Associated diseases and disorders
Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunocytochemistry-immunofluorescence-img106530.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunofluorescence staining of SH-SY5Y cells with purified Anti-Cytochrome C antibody [EPR1327] ab133504 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1 in 500. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 was used to stain tubulin, and this is shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom middle and right hand panels - for the negative controls, purified Anti-Cytochrome C antibody [EPR1327] ab133504 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
![Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunoprecipitation-img43804.jpg "Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Anti-Cytochrome C antibody [EPR1327] ab133504 (purified) at 1/30 immunoprecipitating Cytochrome C in Molt-4 cells (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit (H+L), was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
All lanes: Immunoprecipitation - Anti-Cytochrome C antibody [EPR1327] (Anti-Cytochrome C antibody [EPR1327] ab133504)
Predicted band size: 11 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33698.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Anti-Cytochrome C antibody [EPR1327] ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunohistochemical staining of paraffin embedded mouse liver with purified Anti-Cytochrome C antibody [EPR1327] ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunohistochemical staining of paraffin embedded rat kidney with purified Anti-Cytochrome C antibody [EPR1327] ab133504 at a working dilution of 1 in 500. The secondary antibody used is a HRP goat anti-rabbit H+L (Goat Anti-Rabbit IgG H&L (HRP) ab97051). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
![OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--oi-rd-scanning-img149348.jpg "OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140304.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome C with unpurified Anti-Cytochrome C antibody [EPR1327] ab133504 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunocytochemistry-immunofluorescence-img123131.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)
Immunofluorescent analysis of HeLa cells labelling Cytochrome C with unpurified Anti-Cytochrome C antibody [EPR1327] ab133504 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cytochrome C antibody [EPR1327] ab133504).
![OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--oi-rd-scanning-img149348.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140304.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-c-antibody-epr1327-bsa-and-azide-free-ab218312--immunocytochemistry-immunofluorescence-img123131.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C antibody [EPR1327] - BSA and Azide free (ab218312)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com