AB204790

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody

Name: Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (ab204790) | Abcam
Brand: Abcam Limited
SKU: AB204790
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Cytochrome C Oxidase subunit VIc/COX6C antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Cytochrome c oxidase subunit 6C.

View Alternative Names

Cytochrome c oxidase subunit 6C, Cytochrome c oxidase polypeptide VIc, COX6C

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab204790 antibody at a dilution of 1/200.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue staining Cytochrome C Oxidase subunit VIc (COX6C) with ab204790 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemical analysis of paraffin-embedded human kidney tissue staining Cytochrome C Oxidase subunit VIc (COX6C) with ab204790 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunofluorescent analysis of U-2 OS cells labeling Cytochrome C Oxidase subunit VIc/COX6C with ab204790 at 4 μg/mL (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue staining Cytochrome C Oxidase subunit VIc (COX6C) with ab204790 at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Cytochrome c oxidase subunit 6C. The exact immunogen used to generate this antibody is proprietary information.

P09669

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C Oxidase subunit VIc also known as COX6C or cytochrome c oxidase subunit 1 is a component of the enzyme cytochrome c oxidase also referred to as complex IV in the mitochondrial electron transport chain. This subunit contributes significantly to the mechanical activity by aiding the catalytic conversion of molecular oxygen to water. COX6C is relatively lightweight compared to other subunits with an approximate mass of 7.5 kDa. The expression of COX6C predominantly occurs in tissues with high metabolic activity such as the heart muscle and brain where energetic demands are substantial.

Biological function summary

Cytochrome C Oxidase subunit VIc is part of the larger cytochrome c oxidase complex facilitating efficient electron transfer from cytochrome c to oxygen therefore promoting the process of oxidative phosphorylation. This activity is critical for sustaining the mitochondrial membrane potential and ATP production. COX6C's integration within the complex is necessary for its structural integrity and optimal performance. This subunit impacts mitochondrial functionality playing a pivotal role in cellular energy metabolism.

Pathways

Cytochrome C Oxidase subunit VIc contributes to the oxidative phosphorylation pathway and impacts the electron transport chain's efficiency. Its function within this pathway is interconnected with cytochrome c and other cytochrome oxidase complexes. During this process ATP synthase takes advantage of the proton gradient established by the electron flow managed by complex IV. COX6C's role within these pathways ensures effective energy conversion maintaining cellular respiration and energy output in aerobically respiring cells.

Abnormalities in cytochrome C Oxidase subunit VIc expression or function often lead to mitochondrial diseases such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions are linked with defects in cellular respiration causing a wide array of symptoms including neurological and muscular impairments. The subunit's relationship with cytochrome c integral to both electron transfer and apoptosis pathways highlights its influence in these mitochondrial disorders. Understanding COX6C's role offers insights into potential therapeutic targets to mitigate these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information Cytochrome c oxidase subunit 6C

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:4287 PubMed32855419

2020

Warsaw Breakage Syndrome associated DDX11 helicase resolves G-quadruplex structures to support sister chromatid cohesion.

Applications

Unspecified application

Species

Unspecified reactive species

Janne J M van Schie,Atiq Faramarz,Jesper A Balk,Grant S Stewart,Erika Cantelli,Anneke B Oostra,Martin A Rooimans,Joanna L Parish,Cynthia de Almeida Estéves,Katja Dumic,Ingeborg Barisic,Karin E M Diderich,Marjon A van Slegtenhorst,Mohammad Mahtab,Francesca M Pisani,Hein Te Riele,Najim Ameziane,Rob M F Wolthuis,Job de Lange

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (AB204790)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise