AB150422

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938]

RabMAb
Recombinant
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(12 Publications)

Rabbit Recombinant Monoclonal Cytochrome C Oxidase subunit VIc/COX6C antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

Cytochrome c oxidase subunit 6C, Cytochrome c oxidase polypeptide VIc, COX6C

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin embedded human colon tissue using ab150422 showing positive staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin embedded human colonic adenocarcinoma tissue using ab150422 showing positive staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1 : 500 dilution (0.45 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Cytochrome C Oxidase subunit VIc/COX6C with ab150422 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin embedded human liver tissue using ab150422 showing positive staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using ab150422 showing positive staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1 : 500 dilution (0.45 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cardiac muscle tissue sections labeling Cytochrome C Oxidase subunit VIc/COX6C with purified ab150422 at 1 : 500 dilution (0.45 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

All lanes:

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution

Lane 1:

Human fetal heart cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

Lane 3:

A-673 (Human muscle Ewing's Sarcoma cell line) cell lysate at 10 µg

Predicted band size: 9 kDa

false

Lab

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

All lanes:

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/1000 dilution

Lane 1:

Human heart lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Rat heart lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 9 kDa

Observed band size: 12 kDa

false

Lab

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

All lanes:

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (ab150422) at 1/5000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 9 kDa

Observed band size: 12 kDa

false

Carrier free

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9938

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C Oxidase subunit VIc also known as COX6C or cytochrome c oxidase subunit 1 is a component of the enzyme cytochrome c oxidase also referred to as complex IV in the mitochondrial electron transport chain. This subunit contributes significantly to the mechanical activity by aiding the catalytic conversion of molecular oxygen to water. COX6C is relatively lightweight compared to other subunits with an approximate mass of 7.5 kDa. The expression of COX6C predominantly occurs in tissues with high metabolic activity such as the heart muscle and brain where energetic demands are substantial.

Biological function summary

Cytochrome C Oxidase subunit VIc is part of the larger cytochrome c oxidase complex facilitating efficient electron transfer from cytochrome c to oxygen therefore promoting the process of oxidative phosphorylation. This activity is critical for sustaining the mitochondrial membrane potential and ATP production. COX6C's integration within the complex is necessary for its structural integrity and optimal performance. This subunit impacts mitochondrial functionality playing a pivotal role in cellular energy metabolism.

Pathways

Cytochrome C Oxidase subunit VIc contributes to the oxidative phosphorylation pathway and impacts the electron transport chain's efficiency. Its function within this pathway is interconnected with cytochrome c and other cytochrome oxidase complexes. During this process ATP synthase takes advantage of the proton gradient established by the electron flow managed by complex IV. COX6C's role within these pathways ensures effective energy conversion maintaining cellular respiration and energy output in aerobically respiring cells.

Abnormalities in cytochrome C Oxidase subunit VIc expression or function often lead to mitochondrial diseases such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions are linked with defects in cellular respiration causing a wide array of symptoms including neurological and muscular impairments. The subunit's relationship with cytochrome c integral to both electron transfer and apoptosis pathways highlights its influence in these mitochondrial disorders. Understanding COX6C's role offers insights into potential therapeutic targets to mitigate these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information Cytochrome c oxidase subunit 6C

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer (Tokyo, Japan) 32:988-1005 PubMed40411659

2025

Helix pomatia agglutinin bound to surface glycans of small extracellular vesicles in-vitro and in-vivo increases in early and late stage breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie Cooper,Bethy Airstone,Ellie Beaman,Emanuela Carollo,Susan Ann Brooks,Ryan Charles Pink

Environment & health (Washington, D.C.) 3:58-67 PubMed39839250

2025

Unveiling the Origin of Copper Accumulation in Plasma with Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Weichao Wang,Dawei Lu,Hang Yang,Zigu Chen,Weibo Ling,Shanjun Song,Linyi Peng,Qian Liu,Guibin Jiang

International journal of molecular sciences 24: PubMed37894873

2023

Characterizing Early Cardiac Metabolic Programming via 30% Maternal Nutrient Reduction during Fetal Development in a Non-Human Primate Model.

Applications

Unspecified application

Species

Unspecified reactive species

Susana P Pereira,Mariana S Diniz,Ludgero C Tavares,Teresa Cunha-Oliveira,Cun Li,Laura A Cox,Mark J Nijland,Peter W Nathanielsz,Paulo J Oliveira

Frontiers in endocrinology 14:1131993 PubMed37334311

2023

Changes and related factors of blood CCN1 levels in diabetic patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yu Xiang,Shu-Li Chen,Xin-Ran Qin,Sen-Lin Lin,Yi Xu,Li-Na Lu,Hai-Dong Zou

Cell death & disease 14:82 PubMed36737598

2023

Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingli Liu,Jingyuan Xu,Linyao Lu,Lili Gao,Shengjuan Zhu,Yi Sui,Ting Cao,Tao Yang

Scientific reports 11:14001 PubMed34234233

2021

The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Li,Shuting Liu,Zhenning Dai,Qian Zhang,Yichao Xu,Youyu Chen,Zhenyou Jiang,Wenhua Huang,Hanxiao Sun

Molecular medicine reports 22:5105-5112 PubMed33174030

2020

Effect of TFAM on ATP content in tachypacing primary cultured cardiomyocytes and atrial fibrillation patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yueheng Liu,Ye Zhao,Rui Tang,Xuan Jiang,Yuchao Wang,Tianxiang Gu

Journal of digestive diseases 21:711-723 PubMed33405317

2020

Role of free radical scavenging activity of vasoactive intestinal peptide in the attenuation of mitochondrial dysfunction to ameliorate dextran sulphate sodium-induced colitis in mice: Implications in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Bengaluru Chandraiah Spoorthi,Sunil S More,Santhekadur Annappa Gautham,Shashwati Ghosh,Ishita Saha,Arpan Kumar Maiti

PloS one 13:e0204567 PubMed30252907

2018

Colonic levels of vasoactive intestinal peptide decrease during infection and exogenous VIP protects epithelial mitochondria against the negative effects of IFNγ and TNFα induced during Citrobacter rodentium infection.

Applications

Unspecified application

Species

Unspecified reactive species

Arpan K Maiti,Sinan Sharba,Nazanin Navabi,Sara K Lindén

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 373: PubMed29158318

2017

Cisplatin induces the release of extracellular vesicles from ovarian cancer cells that can induce invasiveness and drug resistance in bystander cells.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Samuel,Laura Ann Mulcahy,Fiona Furlong,Helen O McCarthy,Susan Ann Brooks,Muller Fabbri,Ryan Charles Pink,David Raul Francisco Carter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

Western blot - Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody [EPR9938] (AB150422)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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