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Rabbit Recombinant Monoclonal Cytochrome P450 17A1/CYP17A1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 34 publications.


Images

Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB125022), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB125022), expandable thumbnail
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB125022), expandable thumbnail
  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB125022), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (AB125022), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

5 products for Alternative Product

1 product for Alternative Version

Target data

Function

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable) (PubMed:25301938, PubMed:9452426). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:36640554, PubMed:9452426). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA (PubMed:36640554). Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:25301938, PubMed:27339894). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426).

Alternative names

CYP17, S17AH, CYP17A1, 17-alpha-hydroxyprogesterone aldolase, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Steroid 17-alpha-monooxygenase, Cytochrome P450c17

Recommended products

Rabbit Recombinant Monoclonal Cytochrome P450 17A1/CYP17A1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 34 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6293
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome P450 17A1 also known as CYP17A1 is an essential enzyme in the steroidogenic pathway. Its molecular mass is approximately 57 kDa. It is part of the cytochrome P450 family and shows expression primarily in the adrenal glands and gonads. This enzyme catalyzes two key reactions: the 17α-hydroxylation of pregnenolone and progesterone and the subsequent 1720-lyase reaction. These reactions are critical for the production of steroid hormones such as glucocorticoids and sex steroids.

Biological function summary

CYP17A1 plays a central role in hormone biosynthesis. The enzyme exists as a single protein and does not form part of a larger complex. By facilitating the conversion of progestogens into androgens it directly impacts the balance and levels of steroid hormones in the body. This function supports vital processes like the development of secondary sexual characteristics and the regulation of metabolism therefore affecting multiple physiological functions.

Pathways

CYP17A1 integrates into both the steroidogenesis and biosynthesis pathways of adrenal and sex hormones. It links directly with proteins such as steroidogenic acute regulatory protein (StAR) and cytochrome P450 oxidoreductase (POR) which assist in enabling its enzymatic function. These pathways regulate the levels of hormones influencing biological processes including stress responses and reproductive functions.

Associated diseases and disorders

CYP17A1 is associated with conditions like congenital adrenal hyperplasia and prostate cancer. In congenital adrenal hyperplasia CYP17A1 mutations disrupt the steroidogenesis pathway leading to cortisol production deficiency. In prostate cancer CYP17A1 is often upregulated contributing to increased androgen levels that fuel cancer progression. Proteins such as 3β-hydroxysteroid dehydrogenase (3β-HSD) and androgen receptor (AR) interact with CYP17A1 in these disorders demonstrating its importance in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022), expandable thumbnail

    Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    ab125022 (purified) at 1:30 dilution (2μg) immunoprecipitating Cytochrome P450 17A1/CYP17A1 in Human fetal heart lysate.
    Lane 1 (input): Human fetal heart lysate 10μg
    Lane 2 (+): ab125022 & Human fetal heart lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab125022 in Human fetal heart lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 17A1/CYP17A1 with Purified ab125022 at 1:100 dilution (3.4 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022), expandable thumbnail

    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    All lanes: Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/2000 dilution

    All lanes: Human fetal heart lysates at 15 µg

    Predicted band size: 57 kDa

  • Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022), expandable thumbnail

    Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    All lanes: Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022) at 1/1000 dilution

    Lane 1: Human adrenal gland lysate at 10 µg

    Lane 2: SK-OV-3 lysate at 10 µg

    Lane 3: HeLa lysate at 10 µg

    Lane 4: Jurkat lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

    Observed band size: 55 kDa

  • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytochrome P450 17A1/CYP17A1 with purified ab125022 at 1/220 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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