Mouse Monoclonal Cytochrome P450 1A2 antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide, Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Cyp1a2.
Images
![Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (AB56073), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-p450-1a2-antibody-3b8c1-ab56073--western-blot-img81078.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (AB56073)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (AB56073), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-p450-1a2-antibody-3b8c1-ab56073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35818.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (AB56073)")
Publications
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- The Biochemical journal 478:2163-21782021Identification of the contact region responsible for the formation of the homomeric CYP1A2•CYP1A2 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAratrika Saha,J Patrick Connick,James R Reed,Charles S Lott,Wayne L BackesPubMed 34032264
- International journal of molecular medicine 39:101-1122016A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoqian Zhang,Juan Lu,Bin He,Lingling Tang,Xiaoli Liu,Danhua Zhu,Hongcui Cao,Yingjie Wang,Lanjuan LiPubMed 27959388
- Hepatology research : the official journal of the 46:1045-572016Functional polymer-dependent 3D culture accelerates the differentiation of HepaRG cells into mature hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichiro Higuchi et. al.PubMed 26724677
- Toxicological sciences : an official journal of the Society of Toxicology 137:189-2112013A correlation between the in vitro drug toxicity of drugs to cell lines that express human P450s and their propensity to cause liver injury in humans.Applications:WBReactive species:HumanFrida Gustafsson,Alison J Foster,Sunil Sarda,Matthew H Bridgland-Taylor,J Gerry KennaPubMed 24085192
- Drug metabolism and disposition: the biological fa 37:2087-942009Hepatic cytochrome P450 enzyme alterations in humans with progressive stages of nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCraig D Fisher et. al.PubMed 19651758
- The Journal of pharmacology and experimental thera 330:389-4022009Up-regulation of transporters and enzymes by the vitamin D receptor ligands, 1alpha,25-dihydroxyvitamin D3 and vitamin D analogs, in the Caco-2 cell monolayer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianghong Fan et. al.PubMed 19414624
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.08% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Rat Cyp1a2. Database link P04799
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Synthetic peptide | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:11555828, PubMed:12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed:11555828, PubMed:12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed:19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed:9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854). Metabolizes caffeine via N3-demethylation (Probable).
Alternative names
Cytochrome P450 1A2, CYPIA2, Cholesterol 25-hydroxylase, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3, Hydroperoxy icosatetraenoate dehydratase, CYP1A2
Recommended products
Mouse Monoclonal Cytochrome P450 1A2 antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide, Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Cyp1a2.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.08% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Rat Cyp1a2. Database link P04799
- Clone number
- 3B8C1
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome P450 1A2 often known as CYP1A2 is an important enzyme in the cytochrome P450 superfamily with a known mass around 58 kDa. It is mainly expressed in the liver where it functions in the metabolism of drugs and the bioactivation of various compounds. CYP1A2 also participates in the oxidation of small organic molecules which include xenobiotics and endogenous substrates. Due to its function researchers frequently study CYP1A2 substrates and its inhibitors to understand better its role in drug metabolism and toxicity.
- Biological function summary
CYP1A2 plays a central role in the oxidative biotransformation of drugs and procarcinogens. It does not form part of a larger complex but interacts dynamically with other enzymes in the detoxification process. CYP1A2 metabolizes several clinical drugs such as caffeine and theophylline and regulates the detoxification of aromatic amines and hydrocarbons. Studying CYP1A2 inhibitors can provide insights into clinically relevant drug interactions and potential side effects in pharmacotherapy.
- Pathways
CYP1A2 functions within the drug metabolism and synthesis of cholesterol steroids and other lipids pathways. In the drug metabolism pathway CYP1A2 works alongside other cytochrome P450 enzymes such as CYP1A1 and CYP1B1 which together ensure the metabolism and clearance of various exogenous and endogenous compounds. These pathways are vital for maintaining drug efficacy and preventing toxicity through the metabolic clearance of pharmaceutical agents and harmful substances.
- Associated diseases and disorders
CYP1A2 activity links to liver disorders and certain cancers. Altered enzyme activity may lead to hepatotoxicity a condition that arises from excessive accumulation of active drug metabolites in the liver. Furthermore due to its role in activating procarcinogens increased CYP1A2 activity relates to a higher risk of developing certain cancers including liver cancer. Researchers often explore these connections to better understand the enzyme's role in disease pathogenesis and to develop CYP1A2-directed therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-p450-1a2-antibody-3b8c1-ab56073--western-blot-img81078.jpg "Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)")
Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)
All lanes: Western blot - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)
Lane 2: recombinant CYP1B1 at 0.5pmol/lane
Lane 3: recombinant CYP1A1 at 0.5pmol/lane
Lane 4: recombinant CYP1A2 at 0.5pmol/lane
Predicted band size: 58 kDa
Observed band size: 58 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073), expandable thumbnail](https://content.abcam.com/products/images/cytochrome-p450-1a2-antibody-3b8c1-ab56073--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35818.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [3B8C1] (ab56073)
Immunohistochemical staining of formalin-fixed,
paraffin-embedded normal human liver tissue section
using ab56073.
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