Anti-Cytochrome P450 1A2 antibody [EPR28416-31]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse colon (PMID : 31147315). The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse cerebrum (PMID : 31147315). The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat colon. The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat cerebrum. The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument .Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Rat liver showed expected staining patterns (PMID : 36220068, PMID : 34873187). The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Cytochrome P450 1A2 with ab314666 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mouse liver showed expected staining patterns (PMID : 36220068, PMID : 34873187)). The section was incubated with ab314666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

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Immunoprecipitation - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Cytochrome P450 1A2 was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab314666 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314666 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse liver tissue lysate Lane 2 : ab314666 IP in Mouse liver tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314666 in mouse liver tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (ab314666) at 1/30 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : colon, brain, testis, spleen. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (ab314666) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse testis tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (AB314666)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with mouse CP1A1. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Cytochrome P450 1A2 antibody [EPR28416-31] (ab314666) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse CP1A1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse CP1A2 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa

false

Exposure time: 92s