Anti-Cytochrome P450 1A2 antibody [EPR6138(2)]

6 product images

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  Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  All lanes: Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/2000 dilution

  All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 58 kDa

  Observed band size: 58 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (9.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  All lanes: Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/1000 dilution

  Lane 1: Caco2 cell lysate at 10 µg

  Lane 2: HepG2 cell lysate at 10 µg

  Lane 3: HeLa cell lysate at 10 µg

  Lane 4: A549 cell lysate at 10 µg

  Secondary

  All lanes: Goat anti-rabbit HRP at 1/2000 dilution

  Predicted band size: 58 kDa

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  Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  Immunofluorescent analysis of HeLa cells labeling Cytochrome P450 1A2 with unpurified ab151728 at 1/250 dilution.

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  Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

  Overlay histogram showing MCF7 cells stained with unpurified ab151728 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151728, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.