AB22719

Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)]

Name: Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719) | Abcam
Brand: Abcam Limited
SKU: AB22719

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal Cytochrome P450 2B1 antibody. Suitable for IP and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Cyp2b-1, Cyp2b1, Cytochrome P450 2B1, CYPIIB1, Cytochrome P450-B, Cytochrome P450-LM2, Cytochrome P450-PB1, Cytochrome P450-PB2, Cytochrome P450b

1 Images

Unknown

Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (AB22719)

Cytochrome P450 2B1 + 2B2 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5μg of Mouse monoclonal to Cytochrome P450 2B1 + 2B2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22719.

Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

Band : 56kDa; Cytochrome P450 2B1 + 2B2

All lanes:

Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)

Predicted band size: 56 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

b/e3 (9.14)

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

applications

Specificity

This product does not react with Rat CYP 1A1, 1A2, 2A1, 2C6, 2C7, 2C11, 4A2 and 4A3

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2B1 and 2B2 are enzymes that belong to the cytochrome P450 superfamily often referred to as CYP2B1 and CYP2B2 respectively. They are important in drug metabolism and chemical biotransformation. These enzymes have a role in oxidizing organic substances which helps in processing drugs in the liver. Each has a molecular mass of around 54 kDa and shows expression in tissues primarily in the liver and to some extent in the lungs.

Biological function summary

Cytochrome P450 enzymes including 2B1 and 2B2 function to metabolize endogenous and exogenous compounds through monooxygenase activity. 2B1 and 2B2 do not operate in solitude but are involved with the cytochrome P450 complex which includes NADPH-cytochrome P450 reductase. This complex plays an integral role in enabling the metabolism of a wide variety of substrates such as steroids fatty acids and xenobiotics.

Pathways

Cytochrome P450 2B1 and 2B2 take part in the metabolic pathway of xenobiotics and various drugs. These pathways include hydroxylation processes necessary for drug detoxification and clearance. In this context CYP2B1 and CYP2B2 interact closely with other proteins like CYP3A4 which also plays a part in drug metabolism within the liver to ensure coordinated metabolic conversion of foreign chemicals.

CYP2B1 and CYP2B2 have significance in conditions such as liver disease and certain types of drug-induced toxicity. The enzymes' altered activity can lead to improper drug clearance and toxic buildup contributing to liver disorders. They also have associations with specific proteins like GST (glutathione S-transferases) that aid in detoxification and changes in these interactions may exacerbate susceptibility to drug-related liver damage.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

See full target information Cyp2b1

Additional targets

Cyp2b2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 180:2973-2988 PubMed37403641

2023

Role of circadian clock in the chronoefficacy and chronotoxicity of clopidogrel.

Applications

Unspecified application

Species

Unspecified reactive species

Luyao Ma,Fangjun Yu,Di He,Lianxia Guo,Yu Yang,Wangchun Li,Tianpeng Zhang

Biomedicines 10: PubMed36140274

2022

Ablation of Discoidin Domain Receptor 1 Provokes an Osteopenic Phenotype by Regulating Osteoblast/Osteocyte Autophagy and Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Chiao Chou,Sung-Yen Lin,Liang-Yin Chou,Mei-Ling Ho,Shu-Chun Chuang,Tsung-Lin Cheng,Lin Kang,Yi-Shan Lin,Yan-Hsiung Wang,Chun-Wang Wei,Chung-Hwan Chen,Chau-Zen Wang

International journal of molecular sciences 21: PubMed33003599

2020

Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Yin Chou,Chung-Hwan Chen,Shu-Chun Chuang,Tsung-Lin Cheng,Yi-Hsiung Lin,Hsin-Chiao Chou,Yin-Chih Fu,Yan-Hsiung Wang,Chau-Zen Wang

Toxicology mechanisms and methods 30:562-569 PubMed32508177

2020

Downregulation of by miR-21 may participate in liver fibrosis upon chronic DDT exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Mikhail D Chanyshev,Andrey A Yarushkin,Elena V Koldysheva,Elena L Lushnikova,Lyudmila F Gulyaeva

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)]

Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (AB22719)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise