AB140609

Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)]

Name: Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) | Abcam
Brand: Abcam Limited
SKU: AB140609
Price: 335 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal Cytochrome P450 2B6/CYP2B6 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Cytochrome P450 2B6, CYPIIB6, Cytochrome P450 IIB1, CYP2B6

2 Images

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Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (AB140609)

All lanes:

Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 56 kDa

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Lab

Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (AB140609)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (ab140609) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell from embryonic kidney transformed with large T antigen) transfected with an empty expression vector, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with full length His-tagged Human CYP2B6 expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9985(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2B6 (CYP2B6) an essential enzyme in the cytochrome P450 superfamily plays a role in the metabolism of various substances. Often referred to as CYP2B6 or P450 2B6 this enzyme has a molecular mass of approximately 56 kDa. CYP2B6 is predominantly expressed in the liver but also found in the brain and testes. It catalyzes oxidation reactions thereby affecting the biotransformation of endogenous and exogenous compounds including drugs and steroids.

Biological function summary

CYP2B6 is involved in metabolizing drugs and synthesizing cholesterol steroids and other lipids. It forms part of a broader enzymatic complex that facilitates these biochemical reactions. As a monooxygenase it introduces an oxygen atom into its substrates contributing to drug metabolism and clearance from the body. This function aids detoxification processes and supports homeostasis.

Pathways

CYP2B6 is integral to the metabolism of xenobiotics and drug biotransformation pathways. It closely interacts with other cytochrome P450 enzymes such as CYP3A4 in these pathways. During drug metabolism these enzymes can affect the pharmacokinetics and pharmacodynamics of various pharmaceutical agents influencing their efficacy and toxicity.

CYP2B6 shows a connection with drug-induced liver injury and certain neurological disorders. Genetic polymorphisms in CYP2B6 can affect its function leading to variability in drug metabolism between individuals. This can contribute to adverse drug reactions or altered therapeutic effects. The enzyme's interaction with proteins like CYP2D6 relates to altered responses to antidepressants impacting treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed : 12865317, PubMed : 21289075). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed : 21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed : 12865317, PubMed : 21289075). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed : 11695850, PubMed : 22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed : 11695850).. Allele 2B6*9 : Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

See full target information Cytochrome P450 2B6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Current protocols 4:e1003 PubMed38483112

2024

Use of Lentivirus-Based Method for Establishing TK6 Human Cell Lines Expressing Cytochrome P450 and its Applications in Genotoxicity Testing.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Li,Si Chen,Xiaobo He,Qiangen Wu,Lei Guo,Nan Mei

Nutrition & metabolism 20:40 PubMed37710320

2023

METTL3 exacerbates insulin resistance in hepatocytes by regulating mA modification of cytochrome P450 2B6.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqing Li,Dantong Zhang,Yinan Gao,Peijun Wang,Zejun Wang,Bingyang Zhang,Junjun Liu,Diwen Ye,Wanshan Ma,Sumei Lu

Journal of ethnopharmacology 298:115587 PubMed35934190

2022

Artemisia extracts differ from artemisinin effects on human hepatic CYP450s 2B6 and 3A4 in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ndeye F Kane,Bushra H Kiani,Matthew R Desrosiers,Melissa J Towler,Pamela J Weathers

Oncology reports 41:178-190 PubMed30320358

2018

Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia‑inducible factor 1α‑mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Valencia-Cervantes,Sara Huerta-Yepez,Guillermo Aquino-Jarquín,Sara Rodríguez-Enríquez,Daniel Martínez-Fong,José-Antonio Arias-Montaño,Víctor Manuel Dávila-Borja

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)]

Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (AB140609)

Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody [EPR9985(B)] (AB140609)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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