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AB28146

Anti-Cytochrome P450 2E1 antibody

5

(6 Reviews)

|

(153 Publications)

Anti-Cytochrome P450 2E1 antibody (ab28146) is a rabbit polyclonal antibody detecting Cytochrome P450 2E1 in Western Blot. Suitable for Human, Mouse, Rabbit, Rat.

- Over 150 publications
- Trusted since 2006

View Alternative Names

Cyp2e, Cyp2e-1, Cyp2e1, Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-ALC, Cytochrome P450-J

2 Images
Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)
  • WB

AbReview25310****

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

All lanes:

Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution

All lanes:

Tissue lysate prepared from normal murine liver at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa

true

Exposure time: 3s

Image courtesy of an anonymous Abreview.

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)
  • WB

Unknown

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

All lanes:

Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Cell lysates prepared from human liver microsomes

Lane 3:

Cell lysates prepared from rat liver microsomes

Lane 4:

Cell lysates prepared from mouse liver microsomes

Lane 5:

Cell lysates prepared from rabbit liver microsomes

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Rabbit, Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Rat Cyp2e1.

P05182

Specificity

The antibody detects a ~50-55 kDa protein, corresponding to the apparent molecular mass of cytochrome P450 IIE1 on SDS-PAGE immunoblots.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.</p>" }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p>This dilution was sufficient for detection of Cytochrome P450 in 20ug of human liver microsomes by colorimetric immunoblot analysis using Goat anti-Rabbit IgG:AP as the secondary antibody.</p>" } } }

Product details

What is this antibody validated in?
Anti-Cytochrome P450 2E1 antibody (ab28146) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rabbit, Rat samples.

What is the molecular weight of Cytochrome P450 2E1?
Anti-Cytochrome P450 2E1 (ab28146) specifically detects a band for Cytochrome P450 2E1 (UniProt: P05181) at a molecular weight of 50-55kDa.

Trusted by the scientific community
Anti-Cytochrome P450 2E1 (ab28146) was first used in a scientific publication in 2006 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Cytochrome P450 2E1 (ab28146) has over 5 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2E1 also known as CYP2E1 is an enzyme belonging to the cytochrome P450 superfamily. It weighs approximately 56 kDa. This enzyme is majorly expressed in the liver but can also be found in other tissues like the kidney and lungs. It plays a central role in the oxidation and metabolism of various endogenous and exogenous substances including fatty acids drugs and alcohols.
Biological function summary

The enzyme CYP2E1 metabolizes low-molecular-weight compounds. It catalyzes the conversion of substrates to more soluble forms enabling their excretion from the body. It is not a part of a larger protein complex but often partners with NADPH-cytochrome P450 reductase in electron transfer. CYP2E1 can also produce reactive oxygen species during its catalytic cycle which may impact cellular structures and processes.

Pathways

CYP2E1 is involved in the oxidative metabolism of ethanol and other xenobiotics. It participates in the metabolic pathways for drugs and carcinogens transforming them into either active or inactive metabolites. The enzyme has connections with other P450 enzymes such as CYP3A4 within the same metabolic landscape often overlapping substrates and regulatory mechanisms.

CYP2E1 plays a significant role in alcohol-induced liver injury because its metabolism of ethanol generates toxic by-products like acetaldehyde and reactive oxygen species contributing to liver damage. The enzyme is also associated with the development of certain cancers as its metabolism of procarcinogens can produce carcinogenic metabolites. Other related cytochrome P450 enzymes such as CYP1A2 share pathways involved in carcinogen activation highlighting an interconnected network contributing to disease progression.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
See full target information Cyp2e1

Publications (153)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37891965

2023

Ellagic Acid Prevented Dextran-Sodium-Sulfate-Induced Colitis, Liver, and Brain Injury through Gut Microbiome Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Ha Kim,Ji-Su Kim,Jae-Hee Kwon,In-Sook Kwun,Moon-Chang Baek,Gi-Seok Kwon,Wiramon Rungratanawanich,Byoung-Joon Song,Do-Kyun Kim,Hyo-Jung Kwon,Young-Eun Cho

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301096 PubMed37283464

2023

Identification of Cytochrome P450 2E1 as a Novel Target in Glioma and Development of Its Inhibitor as an Anti-Tumor Agent.

Applications

Unspecified application

Species

Unspecified reactive species

Guiming Hu,Yan Fang,Haiwei Xu,Guanzhe Wang,Rui Yang,Fei Gao,Qingda Wei,Yuhan Gu,Cunzhen Zhang,Jinhuan Qiu,Na Gao,Qiang Wen,Hailing Qiao

Nature genetics 55:651-664 PubMed36914834

2023

Bipotent transitional liver progenitor cells contribute to liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Pu,Huan Zhu,Mingjun Zhang,Monika Pikiolek,Caner Ercan,Jie Li,Xiuzhen Huang,Ximeng Han,Zhenqian Zhang,Zan Lv,Yan Li,Kuo Liu,Lingjuan He,Xiuxiu Liu,Markus H Heim,Luigi M Terracciano,Jan S Tchorz,Bin Zhou

Science advances 9:eabq2937 PubMed36638165

2023

The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Jia,Juan Liu,Tinghe Fang,Zhen Zhou,Ruihong Li,Wenzhen Yin,Yao Qian,Qi Wang,Wanhui Zhou,Chang Liu,Dingcheng Sun,Xun Chen,Zheng Ouyang,Jiahong Dong,Yunfang Wang,Shuhua Yue

Drug development research 84:211-225 PubMed36567664

2022

Oridonin ameliorates acetaminophen-induced acute liver injury through ATF4/PGC-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Yu,Jiye Li,Yu Wang,Danfeng Guo,Xiaodan Zhang,Mingming Chen,Zheng Zhou

Advanced biology 7:e2200208 PubMed36328790

2022

Temporal Dynamics of Metabolic Acquisition in Grafted Engineered Human Liver Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea L Fortin,Tara N McCray,Sarah H Saxton,Fredrik Johansson,Christian B Andino,Jonathan Mene,Yuliang Wang,Kelly R Stevens

Evidence-based complementary and alternative medicine : eCAM 2022:2481654 PubMed36285162

2022

Mechanisms Underlying the Differences in the Pharmacokinetics of Six Active Constituents of Huangqi Liuyi Decoction between Normal and Diabetic Nephropathy Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Wang,Yonglin Wang,Wen Liu,Dingyan Lu,Yang Jin,Nian Tang,Ya Shi,Zipeng Gong,Weiyi Tian,Ting Liu

Journal of the American Society for Mass Spectrometry 33:2094-2107 PubMed36223142

2022

Desorption Electrospray Ionization Mass Spectrometry Imaging Allows Spatial Localization of Changes in Acetaminophen Metabolism in the Liver after Intervention with 4-Methylpyrazole.

Applications

Unspecified application

Species

Unspecified reactive species

Jephte Yao Akakpo,Matthew Wolfgang Jaeschke,Yasaman Etemadi,Antonio Artigues,Scott Toerber,Hernando Olivos,Bindesh Shrestha,Anthony Midey,Hartmut Jaeschke,Anup Ramachandran

International journal of molecular sciences 23: PubMed36232707

2022

Hepatoprotective Effects of a Natural Flavanol 3,3'-Diindolylmethane against CCl-Induced Chronic Liver Injury in Mice and TGFβ1-Induced EMT in Mouse Hepatocytes via Activation of Nrf2 Cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Suvesh Munakarmi,Yamuna Gurau,Juna Shrestha,Prabodh Risal,Ho Sung Park,Hyun Beak Shin,Yeon Jun Jeong

Alcohol (Fayetteville, N.Y.) 107:119-135 PubMed36150611

2022

Profiling the oxylipidome in aged mice after chronic ethanol feeding: Identifying lipid metabolites as drivers of hepatocyte stress.

Applications

Unspecified application

Species

Unspecified reactive species

Paige E Anton,Lauren N Rutt,Courtney Capper,David J Orlicky,Rebecca L McCullough
View all publications

Product promise

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