Anti-Cytochrome P450 2E1 antibody

• WB

AbReview25310****

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

All lanes:

Western blot - Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilution

All lanes:

Tissue lysate prepared from normal murine liver at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 55 kDa

true

Exposure time: 3s

Image courtesy of an anonymous Abreview.

• WB

CiteAb

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

Western Blotting using Anti-Cytochrome P450 2E1 antibody, ab28146. Publication image from Zhong, F. et al., 2019, Cell Res, 31076642. Legend direct from paper.

C3 contributes to the ethanol-induced steatosis by regulating CYP2E1 expression in AFLD mice. a The expression of CYP2E1 in CR2-Crry-treated or C3−/− mice was detected by western blot. b CYP2E1 activity. c, d MDA, GSH and SOD levels in liver samples. eC3−/− mice were administered with the peptide C3a (pC3a) or Asp (pAsp). Saline administration served as the control. The expression of CYP2E1 was detected by western blot. f AAV9-shRNAs were used to knock down C5aR2. The effect of C5aR2 knockdown on the expression of CYP2E1 was examined. g Co-immunoprecipitation of C5aR2 with β-arrestin2. h AML12 cells were transfected with Arrb2 siRNA, and after 12 h treated with 100 mM ethanol. CYP2E1 expression was detected by western blot. NC, negative control for siRNA. i, j AAV9-shRNAs or CMZ was used to downregulate Cyp2e1. The effect of CYP2E1 downregulation on liver steatosis was determined by H&E stainging and liver triglyceride levels. The data are representative of three independent experiments. n.s., not significant. The results are expressed as means ± SD. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001

false

• WB

CiteAb

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

Western Blotting using Anti-Cytochrome P450 2E1 antibody, ab28146. Publication image from Zhong, F. et al., 2019, Cell Res, 31076642. Legend direct from paper.

C3 contributes to the ethanol-induced steatosis by regulating CYP2E1 expression in AFLD mice. a The expression of CYP2E1 in CR2-Crry-treated or C3−/− mice was detected by western blot. b CYP2E1 activity. c, d MDA, GSH and SOD levels in liver samples. eC3−/− mice were administered with the peptide C3a (pC3a) or Asp (pAsp). Saline administration served as the control. The expression of CYP2E1 was detected by western blot. f AAV9-shRNAs were used to knock down C5aR2. The effect of C5aR2 knockdown on the expression of CYP2E1 was examined. g Co-immunoprecipitation of C5aR2 with β-arrestin2. h AML12 cells were transfected with Arrb2 siRNA, and after 12 h treated with 100 mM ethanol. CYP2E1 expression was detected by western blot. NC, negative control for siRNA. i, j AAV9-shRNAs or CMZ was used to downregulate Cyp2e1. The effect of CYP2E1 downregulation on liver steatosis was determined by H&E stainging and liver triglyceride levels. The data are representative of three independent experiments. n.s., not significant. The results are expressed as means ± SD. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001

false

• WB

CiteAb

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

Western Blotting using Anti-Cytochrome P450 2E1 antibody, ab28146. Publication image from Zhong, F. et al., 2019, Cell Res, 31076642. Legend direct from paper.

C3 contributes to the ethanol-induced steatosis by regulating CYP2E1 expression in AFLD mice. a The expression of CYP2E1 in CR2-Crry-treated or C3−/− mice was detected by western blot. b CYP2E1 activity. c, d MDA, GSH and SOD levels in liver samples. eC3−/− mice were administered with the peptide C3a (pC3a) or Asp (pAsp). Saline administration served as the control. The expression of CYP2E1 was detected by western blot. f AAV9-shRNAs were used to knock down C5aR2. The effect of C5aR2 knockdown on the expression of CYP2E1 was examined. g Co-immunoprecipitation of C5aR2 with β-arrestin2. h AML12 cells were transfected with Arrb2 siRNA, and after 12 h treated with 100 mM ethanol. CYP2E1 expression was detected by western blot. NC, negative control for siRNA. i, j AAV9-shRNAs or CMZ was used to downregulate Cyp2e1. The effect of CYP2E1 downregulation on liver steatosis was determined by H&E stainging and liver triglyceride levels. The data are representative of three independent experiments. n.s., not significant. The results are expressed as means ± SD. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001

false

• WB

CiteAb

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

Western Blotting using Anti-Cytochrome P450 2E1 antibody, ab28146. Publication image from Zhong, F. et al., 2019, Cell Res, 31076642. Legend direct from paper.

C3 contributes to the ethanol-induced steatosis by regulating CYP2E1 expression in AFLD mice. a The expression of CYP2E1 in CR2-Crry-treated or C3−/− mice was detected by western blot. b CYP2E1 activity. c, d MDA, GSH and SOD levels in liver samples. eC3−/− mice were administered with the peptide C3a (pC3a) or Asp (pAsp). Saline administration served as the control. The expression of CYP2E1 was detected by western blot. f AAV9-shRNAs were used to knock down C5aR2. The effect of C5aR2 knockdown on the expression of CYP2E1 was examined. g Co-immunoprecipitation of C5aR2 with β-arrestin2. h AML12 cells were transfected with Arrb2 siRNA, and after 12 h treated with 100 mM ethanol. CYP2E1 expression was detected by western blot. NC, negative control for siRNA. i, j AAV9-shRNAs or CMZ was used to downregulate Cyp2e1. The effect of CYP2E1 downregulation on liver steatosis was determined by H&E stainging and liver triglyceride levels. The data are representative of three independent experiments. n.s., not significant. The results are expressed as means ± SD. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001

false