Anti-Cytochrome P450 2E1 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody (AB53945)

IHC image of ab53945 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53945, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

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Western blot - Anti-Cytochrome P450 2E1 antibody (AB53945)

All lanes:

Western blot - Anti-Cytochrome P450 2E1 antibody (ab53945) at 1/1000 dilution

Lane 1:

Control kidney microsome at 10 µg

Lane 2:

Pyridine-treated kidney microsome at 10 µg

Predicted band size: 57 kDa

Observed band size: 57 kDa

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• WB

CiteAb

Western blot - Anti-Cytochrome P450 2E1 antibody (AB53945)

Cytochrome P450 2E1 western blot using anti-Cytochrome P450 2E1 antibody ab53945. Publication image and figure legend from Santra, S., Bishnu, D., et al., 2020, PLoS One, PubMed 32735603.

ab53945 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab53945 please see the product overview.

Activities of CYP2E1 and NOX and their protein expression in LX2 cells at different hours of INH exposure.LX2 cells were incubated with or without INH for 24, 48 and 72 hours (h). Further, data at 0 hour before INH treatment was also depicted in the figure. LX2 cells without INH treatment served as control. (A) Determination of CYP2E1 activity (pmol p-nitrocatechol/min/mg of protein) was performed. *p<0.01 compared to 48 h control cells; **p<0.01 compared to 24 h INH treated group and #p<0.001 compared to 72 h control cells [n = 6]. (B) Western blot of CYP2E1 protein in the cell lysates in response to INH treatment was carried out at various intervals. β-actin was used as in house control [n = 5]. (C) NOX activity [n = 6] and (D) protein expression of NOX1, NOX2 and NOX4 in the cell lysates with INH treatment (T) and without INH treatment (C) at various time periods were detected by Western blot [n = 5]. β-actin was used as in house control. *p<0.01 compared to 48 h control cells; **p<0.001 compared to 72 h control cells and #p<0.01 compared to 48 h INH treated group.

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