Anti-Cytochrome P450 4A/CYP4A11 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of H-4-II-E (rat) cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• WB

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Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (AB3573)

Chemiluminescent detection

All lanes:

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 25 µg

Lane 2:

H-4-II-E cell lysate at 25 µg

Lane 3:

HeLa cell lysate at 25 µg

Predicted band size: 59 kDa

Observed band size: 58 kDa

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