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Rabbit Recombinant Monoclonal Cytochrome P450 4A/CYP4A11 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 7 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Expected
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Rat
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid (PubMed:10553002, PubMed:10660572). Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:15611369). Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:10620324, PubMed:10660572, PubMed:15611369). Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency (PubMed:7679927). May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed:18182499). Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer (PubMed:15145985). Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids (PubMed:18065749). Has little activity toward prostaglandins A1 and E1 (PubMed:7679927).

Alternative names

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Rabbit Recombinant Monoclonal Cytochrome P450 4A/CYP4A11 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 7 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8276

Purification technique

Affinity purification Protein A

Specificity

The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

Cytochrome P450 enzymes like CYP4A11 contribute significantly to the metabolism of long-chain fatty acids. These metabolic processes generate substances important for cell signaling such as hydroxyeicosatetraenoic acids (HETEs). The enzyme also participates indirectly in blood pressure regulation through these metabolic products. Although CYP4A11 does not form part of a protein complex the metabolic byproducts have a role in several cellular mechanisms.

Activity summary

Cytochrome P450 4A also known as CYP4A11 plays an important role in the metabolism of fatty acids and arachidonic acid. This enzyme is a member of the cytochrome P450 superfamily and possesses a molecular mass of approximately 58 kDa. Expression of CYP4A11 occurs mainly in the liver and kidneys. It functions by introducing oxygen into various substrates facilitating their breakdown and further processing. CYP4A11 also exists as a monomer and does not require assembly into larger complexes to function efficiently.

Pathways

CYP4A11 is integral to the fatty acid signaling pathway. Its action leads to the production of 20-HETE a metabolite involved in regulating vascular tone and renal function. Related proteins in this pathway include other cytochrome P450 family members particularly those involved in arachidonic acid metabolism like CYP2E1. Although not directly a part CYP4A11's metabolic activities feed into the pathways that control blood flow and pressure closely linking it to cardiovascular mechanisms.

Associated diseases and disorders

CYP4A11's activity connects to hypertension and atherosclerosis. Dysregulation of its metabolic processes can lead to imbalances in fatty acid derivatives contributing to these conditions. Studies indicated a link between CYP4A11 and cardiovascular diseases with alterations in enzyme activity affecting blood pressure. The protein links to other P450 enzymes that similarly metabolize fatty acids and participate in maintaining cardiovascular health. Notably CYP4A11's influence on hypertension makes it a target for research into antihypertensive therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    All lanes: Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635) at 1/1000 dilution

    Lane 1: Mouse liver lysate at 10 µg

    Lane 2: Rat liver lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human fetal liver lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

    Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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