AB140635

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276]

RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Cytochrome P450 4A/CYP4A11 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 9 publications.

View Alternative Names

CYP4A2, CYP4A11, Cytochrome P450 4A11, 20-hydroxyeicosatetraenoic acid synthase, CYP4AII, CYPIVA11, Cytochrome P-450HK-omega, Cytochrome P450HL-omega, Fatty acid omega-hydroxylase, Lauric acid omega-hydroxylase, Long-chain fatty acid omega-monooxygenase, 20-HETE synthase

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

WB

Unknown

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (AB140635)

All lanes:

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 10 µg

Lane 2:

Rat liver lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

false

Carrier free

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8276

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 4A also known as CYP4A11 plays an important role in the metabolism of fatty acids and arachidonic acid. This enzyme is a member of the cytochrome P450 superfamily and possesses a molecular mass of approximately 58 kDa. Expression of CYP4A11 occurs mainly in the liver and kidneys. It functions by introducing oxygen into various substrates facilitating their breakdown and further processing. CYP4A11 also exists as a monomer and does not require assembly into larger complexes to function efficiently.

Biological function summary

Cytochrome P450 enzymes like CYP4A11 contribute significantly to the metabolism of long-chain fatty acids. These metabolic processes generate substances important for cell signaling such as hydroxyeicosatetraenoic acids (HETEs). The enzyme also participates indirectly in blood pressure regulation through these metabolic products. Although CYP4A11 does not form part of a protein complex the metabolic byproducts have a role in several cellular mechanisms.

Pathways

CYP4A11 is integral to the fatty acid signaling pathway. Its action leads to the production of 20-HETE a metabolite involved in regulating vascular tone and renal function. Related proteins in this pathway include other cytochrome P450 family members particularly those involved in arachidonic acid metabolism like CYP2E1. Although not directly a part CYP4A11's metabolic activities feed into the pathways that control blood flow and pressure closely linking it to cardiovascular mechanisms.

CYP4A11's activity connects to hypertension and atherosclerosis. Dysregulation of its metabolic processes can lead to imbalances in fatty acid derivatives contributing to these conditions. Studies indicated a link between CYP4A11 and cardiovascular diseases with alterations in enzyme activity affecting blood pressure. The protein links to other P450 enzymes that similarly metabolize fatty acids and participate in maintaining cardiovascular health. Notably CYP4A11's influence on hypertension makes it a target for research into antihypertensive therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed : 10553002, PubMed : 10660572, PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 10553002, PubMed : 10660572, PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order : dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid (PubMed : 10553002, PubMed : 10660572). Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed : 10620324, PubMed : 10660572, PubMed : 15611369). Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency (PubMed : 7679927). May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed : 18182499). Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer (PubMed : 15145985). Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids (PubMed : 18065749). Has little activity toward prostaglandins A1 and E1 (PubMed : 7679927).

See full target information CYP4A11

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:25 PubMed40097405

2025

A decrease in Flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Lingru Li,Tianxing Li,Xue Liang,Linghui Zhu,Yini Fang,Ling Dong,Yi Zheng,Xiaoxue Xu,Mingrui Li,Tianqi Cai,Fufangyu Zhao,Meiling Xin,Mingyan Shao,Yuanyuan Guan,Meiyi Liu,Fangli Li,Chenhong Zhang,Qi Wang,Wenlong Sun,Yanfei Zheng

International journal of nanomedicine 19:4103-4120 PubMed38736658

2024

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Jakic,Michal Selc,Filip Razga,Veronika Nemethova,Petra Mazancova,Filip Havel,Michal Sramek,Monika Zarska,Jan Proska,Vlasta Masanova,Iveta Uhnakova,Peter Makovicky,Marta Novotova,Vit Vykoukal,Andrea Babelova

Molecules (Basel, Switzerland) 28: PubMed36677577

2023

Icariin Alleviates Nonalcoholic Fatty Liver Disease in Polycystic Ovary Syndrome by Improving Liver Fatty Acid Oxidation and Inhibiting Lipid Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Hai,Ling Zuo,Meng Wang,Ruoyu Zhang,Munan Wang,Li Ren,Congwen Yang,Jianwei Wang

Journal of dairy science 104:6399-6414 PubMed33773784

2021

Trans triacylglycerols from dairy products and industrial hydrogenated oil exhibit different effects on the function of human umbilical vein endothelial cells via modulating phospholipase A2/arachidonic acid metabolism pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wei,Ruo-Lin Zhou,Ting Luo,Ze-Yuan Deng,Jing Li

Science advances 6: PubMed32917715

2020

Gadofullerene inhibits the degradation of apolipoprotein B100 and boosts triglyceride transport for reversing hepatic steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhou,Mingming Zhen,Meilan Yu,Xue Li,Tong Yu,Jingchao Liu,Wang Jia,Shuai Liu,Lei Li,Jie Li,Zihao Sun,Zhongpu Zhao,Xinyao Wang,Xiaoyan Zhang,Chunru Wang,Chunli Bai

Journal of Cancer 11:1478-1485 PubMed32047554

2020

Alteration of CYP4A11 expression in renal cell carcinoma: diagnostic and prognostic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Sup Kim,Jin Man Kim,Hyo Jin Lee,Jae Sung Lim,In-Ock Seong,Kyung-Hee Kim

Molecular medicine reports 19:4673-4684 PubMed30957185

2019

Effects of daphnetin on lipid metabolism, insulin resistance and oxidative stress in OA‑treated HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yayun Liu,Lu Liao,Yong Chen,Fengmei Han

Drug metabolism and disposition: the biological fa 46:503-513 PubMed29514828

2018

Physiological Regulation of Drug Metabolism and Transport: Pregnancy, Microbiome, Inflammation, Infection, and Fasting.

Applications

Unspecified application

Species

Unspecified reactive species

Edward T Morgan,Joseph L Dempsey,Sylvie M Mimche,Tracey J Lamb,Supriya Kulkarni,Julia Yue Cui,Hyunyoung Jeong,Angela L Slitt

Pharmacology research & perspectives 2:e00027 PubMed24707356

2014

Selective effects of a therapeutic protein targeting tumor necrosis factor-alpha on cytochrome P450 regulation during infectious colitis: Implications for disease-dependent drug-drug interactions.

Applications

WB

Species

Unspecified reactive species

Beatrice A Nyagode,Roya Jahangardi,Matthew D Merrell,Malú G Tansey,Edward T Morgan

View all publications

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