AB13513

Anti-Cytochrome P450 Reductase antibody

Name: Anti-Cytochrome P450 Reductase antibody (ab13513) | Abcam
Brand: Abcam Limited
SKU: AB13513
Price: 585 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(27 Publications)

Rabbit Polyclonal Cytochrome P450 Reductase antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 27 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Por.

View Alternative Names

CYPOR, POR, NADPH--cytochrome P450 reductase, CPR, P450R

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (AB13513)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (AB13513)

ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Cytochrome P450 Reductase antibody (AB13513)

Western blot analysis of rat PC12 cell lysate, probed with ab13513.

All lanes:

Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution

All lanes:

Cell lysates prepared from rat PC12 cells

Predicted band size: 76 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Native Full Length Protein corresponding to Rat Por.

P00388

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 reductase also known as NADPH--cytochrome P450 reductase (CPR) is an enzyme important in electron transfer. It has a mass of approximately 77 kDa and is responsible for transferring electrons from NADPH to cytochrome P450 enzymes supporting their function. The enzyme is predominantly expressed in the endoplasmic reticulum of liver cells and other tissues where detoxification processes occur. Cytochrome reductase as it is sometimes called plays a fundamental role in the metabolism of drugs and the synthesis of cholesterol steroids and other lipids.

Biological function summary

CPR facilitates electron donation to all microsomal cytochrome P450 enzymes which are involved in a variety of oxidative reactions. This protein is essential for P450 activity assay as it is part of a multienzyme complex that metabolizes exogenous and endogenous compounds efficiently. Without cytochrome P450 reductase the catalytic activity of cytochrome P450 enzymes becomes impaired affecting metabolizing mechanisms for various substances and having systemic biological impacts.

Pathways

Cytochrome P450 reductase directly participates in drug metabolism and steroid biosynthesis pathways. It together with the cytochrome P450 enzymes metabolizes drugs to make them more water-soluble for excretion. The proteins 3 beta-hydroxysteroid dehydrogenase and CYP3A4 are some of the related components within these pathways working in conjunction to achieve metabolic homeostasis. P450 reductase assays are often utilized to study these pathways further understanding the biological roles and interactions of these proteins.

CPR has a significant connection to conditions like congenital adrenal hyperplasia and prostate cancer. Mutations or dysregulations in cytochrome P450 reductase can lead to accumulated steroid precursors and disrupted steroid hormone synthesis contributing to these disorders. The relationship with adrenal enzyme 21-hydroxylase and the androgen receptor illustrates the pathway complexities and the clinical relevance of cytochrome P450 reductase. Understanding these connections highlights the importance of CPR in maintaining normal physiological functions and how its abnormalities can lead to serious health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

See full target information POR

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:105035 PubMed37442234

2023

Mitochondrial cytochrome P450 1B1 is involved in pregnenolone synthesis in human brain cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqi Christina Lin,Garett Cheung,Zeyu Zhang,Vassilios Papadopoulos

Molecular carcinogenesis 62:249-260 PubMed36321415

2022

POR overexpression induces tamoxifen-resistance in breast cancer through the STAT1/c-Myc pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Si Chen,Dingjie Wu,Qiannan Liu,Feng Jin,Fan Yao,Yue Fang

The Journal of biological chemistry 298:102110 PubMed35688208

2022

The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqi Christina Lin,Garett Cheung,Edith Porter,Vassilios Papadopoulos

Small methods 5:e2100632 PubMed34927948

2021

Engineering Tissue-Specific, Multiscale Microvasculature with a Capillary Network for Prevascularized Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Jeonghyun Son,Sung Joon Hong,Jun Woo Lim,Wonwoo Jeong,Jae Hyun Jeong,Hyun-Wook Kang

Scientific reports 10:14193 PubMed32843676

2020

Heterologous expression of high-activity cytochrome P450 in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Kumondai,Eiji Hishinuma,Evelyn Marie Gutiérrez Rico,Akio Ito,Yuya Nakanishi,Daisuke Saigusa,Noriyasu Hirasawa,Masahiro Hiratsuka

mBio 11: PubMed32788383

2020

A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tamutenda Chidawanyika,Kenneth M K Mark,Surachai Supattapone

Clinical cancer research : an official journal of 25:7162-7174 PubMed31527169

2019

Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Fieke E M Froeling,Manojit Mosur Swamynathan,Astrid Deschênes,Iok In Christine Chio,Erin Brosnan,Melissa A Yao,Priya Alagesan,Matthew Lucito,Juying Li,An-Yun Chang,Lloyd C Trotman,Pascal Belleau,Youngkyu Park,Harry A Rogoff,James D Watson,David A Tuveson

Gastroenterology 155:1967-1970.e6 PubMed30170115

2018

Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Francis P Pankowicz,Mercedes Barzi,Kang Ho Kim,Xavier Legras,Celeste Santos Martins,Clavia Ruth Wooton-Kee,William R Lagor,Juan C Marini,Sarah H Elsea,Beatrice Bissig-Choisat,David D Moore,Karl-Dimiter Bissig

Molecular medicine reports 17:6997-7004 PubMed29568933

2018

Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyi Yao,Jin-Zhong Huang,Yingqi Chen,He-Juan Hu,Xiying Tang,Xianhong Li

Molecules (Basel, Switzerland) 22: PubMed29143794

2017

Berberine Activates Aryl Hydrocarbon Receptor but Suppresses CYP1A1 Induction through miR-21-3p Stimulation in MCF-7 Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Nan Lo,Chun-Wei Wang,Yueh-Shieh Chen,Chiung-Chiao Huang,Tian-Shung Wu,Lih-Ann Li,I-Jung Lee,Yune-Fang Ueng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cytochrome P450 Reductase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (AB13513)

Western blot - Anti-Cytochrome P450 Reductase antibody (AB13513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

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