Anti-Cytochrome P450 Reductase antibody [EPR14479(B)]
- BOND RX™ Validated
- RabMAb
- Recombinant
- KO Validated
- 20ul selling size
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(12 Publications)
Rabbit Recombinant Monoclonal Cytochrome P450 Reductase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.
View Alternative Names
CYPOR, POR, NADPH--cytochrome P450 reductase, CPR, P450R
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunohistochemical analysis of paraffin embedded Human infiltrating duct carcinoma of breast tissue labeling Cytochrome P450 Reductase with ab180597 at a 1/150 dilution. HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunofluorescence analysis of MCF7 cells (fixative 4% paraformaldehyde) labeling Cytochrome P450 Reductase with ab180597 at a 1/100 dilution (green), and counterstained with Dapi (blue). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Intracellular Flow Cytometry analysis of HeLa cells using ab180597 at a 1/10 dilution (red) and a rabbit IgG as negative control (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling Cytochrome P450 Reductase with purified ab180597 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunofluorescence analysis of Hela cells (fixative 4% paraformaldehyde) labeling Cytochrome P450 Reductase with ab180597 at a 1/100 dilution (red), and counterstained with Dapi (blue). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Cytochrome P450 Reductase with ab180597 at 1/1000 (0.1 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Postive staining on rat kidney. The section was incubated with ab180597 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Cytochrome P450 Reductase with ab180597 at 1/1000 (0.1 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Postive staining on mouse kidney. The section was incubated with ab180597 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- WB
Lab
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
ab180597 Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] was shown to specifically react with Cytochrome P450 Reductase in HeLa wild-type cells ( ab255448). Loss of signal was observed when knockout cell line ab264996 (knockout cell lysate ab257595) was used. Wild-type and Cytochrome P450 Reductase knockout samples were subjected to SDS-PAGE. ab180597 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/10000 dilution
Lane 1:
Wild-type HeLa lysate at 20 µg
Lane 2:
Cytochrome P450 Reductase knockout HeLa lysate at 20 µg
Lane 2:
Western blot - Human POR (Cytochrome P450 Reductase) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-por-cytochrome-p450-reductase-knockout-hela-cell-line-ab264996'>ab264996</a>)
Lane 3:
HepG2 lysate at 20 µg
Lane 4:
A431 lysate at 20 µg
Predicted band size: 76 kDa
false
- WB
Lab
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Lanes 1- 4 : Merged signal (red and green). Green - ab180597 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180597 was shown to react with Cytochrome P450 Reductase in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266889 (knockout cell lysate ab257596) was used. Wild-type HCT116 and POR knockout HCT116 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180597 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/1000 dilution
Lane 1:
Wild-type HCT116 cell lysate at 40 µg
Lane 2:
POR knockout HCT116 cell lysate at 40 µg
Lane 2:
Western blot - Human POR (Cytochrome P450 Reductase) knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-por-cytochrome-p450-reductase-knockout-hct116-cell-line-ab266889'>ab266889</a>)
Lane 3:
HepG2 cell lysate at 40 µg
Lane 4:
A431 cell lysate at 40 µg
Predicted band size: 76 kDa
Observed band size: 80 kDa
false
- WB
Supplier Data
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/10000 dilution
Lane 1:
HeLa lysate at 20 µg
Lane 2:
MCF7 lysate at 20 µg
Lane 3:
A431 lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 76 kDa
false
- WB
Lab
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1-3 : 26 seconds; Lane 4-7 : 75 seconds. The identities of the lower MW bands between 37 and 60kDa are unknown.
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/1000 dilution
Lane 1:
Mouse brain tissue lysate at 20 µg
Lane 2:
Mouse heart tissue lysate at 20 µg
Lane 3:
Mouse spleen tissue lysate at 20 µg
Lane 4:
Mouse kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 75 kDa
false
- WB
Lab
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1-3 : 26 seconds; Lane 4-7 : 75 seconds. The identities of the lower MW bands between 37 and 60kDa are unknown.
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/1000 dilution
Lane 1:
Rat brain tissue lysate at 20 µg
Lane 2:
Rat heart tissue lysate at 20 µg
Lane 3:
Rat spleen tissue lysate at 20 µg
Lane 4:
Rat kidney tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 75 kDa
false
- WB
Supplier Data
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)
All lanes:
Western blot - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (ab180597) at 1/50000 dilution
All lanes:
HepG2 lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 76 kDa
false
Related conjugates and formulations (2)
-
Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] - BSA and Azide free
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Cytochrome P450 Reductase antibody [EPR14479(B)]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CPR facilitates electron donation to all microsomal cytochrome P450 enzymes which are involved in a variety of oxidative reactions. This protein is essential for P450 activity assay as it is part of a multienzyme complex that metabolizes exogenous and endogenous compounds efficiently. Without cytochrome P450 reductase the catalytic activity of cytochrome P450 enzymes becomes impaired affecting metabolizing mechanisms for various substances and having systemic biological impacts.
Pathways
Cytochrome P450 reductase directly participates in drug metabolism and steroid biosynthesis pathways. It together with the cytochrome P450 enzymes metabolizes drugs to make them more water-soluble for excretion. The proteins 3 beta-hydroxysteroid dehydrogenase and CYP3A4 are some of the related components within these pathways working in conjunction to achieve metabolic homeostasis. P450 reductase assays are often utilized to study these pathways further understanding the biological roles and interactions of these proteins.
Product protocols
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Target data
Publications (12)
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Nature 645:1011-1019 PubMed40804518
2025
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Biological & pharmaceutical bulletin 47:1422-1428 PubMed39111864
2024
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Science advances 10:eadk7329 PubMed38489367
2024
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Cellular and molecular gastroenterology and hepatology 13:1649-1672 PubMed35202887
2022
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International journal of molecular sciences 23: PubMed35054909
2022
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Cell chemical biology 29:157-170.e6 PubMed34813762
2021
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PLoS pathogens 17:e1009927 PubMed34516571
2021
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Nature chemical biology 17:501 PubMed33654315
2021
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Molecular cell 81:355-369.e10 PubMed33321093
2020
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Translational psychiatry 9:260 PubMed31624233
2019
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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