JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB127028

Anti-Cytoglobin antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Cytoglobin antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYGB aa 1 to C-terminus.

View Alternative Names

STAP, CYGB, Cytoglobin, Histoglobin, Nitric oxygen dioxygenase CYGB, Nitrite reductase CYGB, Pseudoperoxidase CYGB, Stellate cell activation-associated protein, Superoxide dismutase CYGB, HGb, NOD

2 Images
Western blot - Anti-Cytoglobin antibody (AB127028)
  • WB

Unknown

Western blot - Anti-Cytoglobin antibody (AB127028)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cytoglobin antibody (ab127028) at 1/2000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Western blot - Anti-Cytoglobin antibody (AB127028)
  • WB

Unknown

Western blot - Anti-Cytoglobin antibody (AB127028)

12% SDS-PAGE

All lanes:

Western blot - Anti-Cytoglobin antibody (ab127028) at 1/1000 dilution

All lanes:

Mouse heart whole cell lysate at 50 µg

Predicted band size: 21 kDa,23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CYGB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WWM9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Store undiluted.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytoglobin also known as CYGB is a globin protein with a molecular weight of approximately 21 kDa. This protein is part of the vertebrate globin family and exhibits oxygen-binding properties. Cytoglobin is mainly expressed in fibroblasts and is detectable in various tissues including the liver brain and kidney. It functions as an intracellular hemoprotein which allows it to facilitate oxygen transport and storage similar to other globins like hemoglobin and myoglobin.
Biological function summary

This globin plays a role in cellular protective mechanisms. Cytoglobin participates in protecting cells from hypoxic and oxidative stress thereby contributing to oxygen homeostasis. Although it is not part of a known protein complex its ability to bind oxygen and reactive oxygen species indicates its significant role in mitigating cellular damage. By maintaining a balance of oxygen levels inside cells cytoglobin helps preserve cellular integrity under stressful conditions.

Pathways

Cytoglobin interacts in significant biological processes related to oxygen regulation. It is closely involved in the hypoxia response pathway where it aids in adapting cells to low oxygen environments. Cytoglobin operates alongside proteins such as hypoxia-inducible factors (HIFs) which help regulate gene expression in response to oxygen availability. It also plays a role in the oxidative stress response connecting to systems involving antioxidant proteins that help mitigate the effects of reactive oxygen species.

Cytoglobin has links to conditions like cancer and fibrotic diseases. Its role in oxygen regulation and response to hypoxia makes it relevant in tumor biology as tumors often create hypoxic environments that promote cancer progression. Cytoglobin's connection to cancer involves interactions with hypoxia-inducible factors that drive tumor adaptability. Additionally in fibrotic diseases such as liver fibrosis cytoglobin's expression levels correlate with disease severity suggesting it as a marker in fibrosis progression. This relation implies potential interactions with proteins like transforming growth factor-beta (TGF-β) which plays a role in fibrotic tissue development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability (PubMed : 11893755, PubMed : 12359339, PubMed : 15165856, PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819, PubMed : 29128400, PubMed : 33576020, PubMed : 34930834). Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption (PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819). By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone (PubMed : 19147491, PubMed : 28393874). Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide (PubMed : 29128400). Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress (PubMed : 12359339, PubMed : 33576020, PubMed : 34930834). Also binds dioxygen with low affinity and could function as an oxygen sensor but has probably no function as a respiratory oxygen carrier (PubMed : 11893755, PubMed : 15299006, PubMed : 20553503).
See full target information CYGB
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Heart and circulat 307:H976-86 PubMed25108012

2014

Role of blood and vascular smooth muscle in the vasoactivity of nitrite.

Applications

Unspecified application

Species

Unspecified reactive species

Taiming Liu,Hobe J Schroeder,Lisa Barcelo,Shannon L Bragg,Michael H Terry,Sean M Wilson,Gordon G Power,Arlin B Blood
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB202972

Anti-Cytoglobin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytoglobin antibody (AB202972)

  • 1
  • 1

Complementary Products

Assay Kits

AB277460

Human Cytoglobin ELISA

ELISA - Human Cytoglobin ELISA (AB277460)

  • 0
  • 0
Primary Antibodies

AB200645

Anti-EDEM1 antibody

Western blot - Anti-EDEM1 antibody (AB200645)

  • 4
  • 1
Primary Antibodies

AB5634

Anti-c-Kit (phospho Y823) antibody

Immunocytochemistry - Anti-c-Kit (phospho Y823) antibody (AB5634)

  • 0
  • 0
Primary Antibodies

AB172509

Anti-CIKS/ACT1 antibody

Western blot - Anti-CIKS/ACT1 antibody (AB172509)

  • 0
  • 0
Primary Antibodies

AB275019

Anti-Chromogranin C/SGII antibody [EPR23876-52]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com