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AB202972

Anti-Cytoglobin antibody

1

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Cytoglobin antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYGB aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

STAP, CYGB, Cytoglobin, Histoglobin, Nitric oxygen dioxygenase CYGB, Nitrite reductase CYGB, Pseudoperoxidase CYGB, Stellate cell activation-associated protein, Superoxide dismutase CYGB, HGb, NOD

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytoglobin antibody (AB202972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytoglobin antibody (AB202972)

Immunohistochemical analysis of parrafin embedded rat brain tissue labeling cytoglobin with ab202972 at 1 : 200, followed by secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytoglobin antibody (AB202972)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytoglobin antibody (AB202972)

Immunohistochemical analysis of parrafin embedded rat colon tissue labeling cytoglobin with ab202972 at 1 : 200, followed by secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CYGB aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8WWM9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytoglobin also known as CYGB is a globin protein with a molecular weight of approximately 21 kDa. This protein is part of the vertebrate globin family and exhibits oxygen-binding properties. Cytoglobin is mainly expressed in fibroblasts and is detectable in various tissues including the liver brain and kidney. It functions as an intracellular hemoprotein which allows it to facilitate oxygen transport and storage similar to other globins like hemoglobin and myoglobin.
Biological function summary

This globin plays a role in cellular protective mechanisms. Cytoglobin participates in protecting cells from hypoxic and oxidative stress thereby contributing to oxygen homeostasis. Although it is not part of a known protein complex its ability to bind oxygen and reactive oxygen species indicates its significant role in mitigating cellular damage. By maintaining a balance of oxygen levels inside cells cytoglobin helps preserve cellular integrity under stressful conditions.

Pathways

Cytoglobin interacts in significant biological processes related to oxygen regulation. It is closely involved in the hypoxia response pathway where it aids in adapting cells to low oxygen environments. Cytoglobin operates alongside proteins such as hypoxia-inducible factors (HIFs) which help regulate gene expression in response to oxygen availability. It also plays a role in the oxidative stress response connecting to systems involving antioxidant proteins that help mitigate the effects of reactive oxygen species.

Cytoglobin has links to conditions like cancer and fibrotic diseases. Its role in oxygen regulation and response to hypoxia makes it relevant in tumor biology as tumors often create hypoxic environments that promote cancer progression. Cytoglobin's connection to cancer involves interactions with hypoxia-inducible factors that drive tumor adaptability. Additionally in fibrotic diseases such as liver fibrosis cytoglobin's expression levels correlate with disease severity suggesting it as a marker in fibrosis progression. This relation implies potential interactions with proteins like transforming growth factor-beta (TGF-β) which plays a role in fibrotic tissue development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability (PubMed : 11893755, PubMed : 12359339, PubMed : 15165856, PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819, PubMed : 29128400, PubMed : 33576020, PubMed : 34930834). Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption (PubMed : 19147491, PubMed : 20511233, PubMed : 28393874, PubMed : 28671819). By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone (PubMed : 19147491, PubMed : 28393874). Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide (PubMed : 29128400). Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress (PubMed : 12359339, PubMed : 33576020, PubMed : 34930834). Also binds dioxygen with low affinity and could function as an oxygen sensor but has probably no function as a respiratory oxygen carrier (PubMed : 11893755, PubMed : 15299006, PubMed : 20553503).
See full target information CYGB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e16235 PubMed36652375

2023

Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Min Wang,Pingyuan Wang,Xiao-Min Chen,Hui Yang,Shan-Shan Song,Zilan Song,Li Jia,Hua-Dong Chen,Xu-Bin Bao,Ne Guo,Xia-Juan Huan,Yong Xi,Yan-Yan Shen,Xin-Ying Yang,Yi Su,Yi-Ming Sun,Ying-Lei Gao,Yi Chen,Jian Ding,Jing-Yu Lang,Ze-Hong Miao,Ao Zhang,Jin-Xue He

Respiratory research 22:52 PubMed33579290

2021

Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Fu,Chunyan Yang,Bing Chen,Kexing Zeng,Siyuan Chen,Yi Fu
View all publications
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