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AB249150

Anti-Cytohesin 1 antibody [EPR10050] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cytohesin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

D17S811E, PSCD1, CYTH1, Cytohesin-1, SEC7 homolog B2-1

1 Images
Western blot - Anti-Cytohesin 1 antibody [EPR10050] - BSA and Azide free (AB249150)
  • WB

Unknown

Western blot - Anti-Cytohesin 1 antibody [EPR10050] - BSA and Azide free (AB249150)

This data was developed using ab154847, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytohesin 1 antibody [EPR10050] (<a href='/en-us/products/primary-antibodies/cytohesin-1-antibody-epr10050-ab154847'>ab154847</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HuT-78 cell lysate at 10 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10050

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab249150 is the carrier-free version of ab154847.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytohesin 1 also known as CYTH1 or PSCD1 belongs to the cytohesin family. It plays a role as a guanine nucleotide exchange factor (GEF) specifically for Arf (ADP-ribosylation factor) proteins. Cytohesin 1 weighs approximately 47 kDa. It contributes to cellular communication and membrane trafficking activities by activating Arf family GTPases. You can find it widely expressed in tissues including immune cells and the nervous system. It localizes to cytoplasmic regions and associates with the plasma membrane through a pleckstrin homology (PH) domain.
Biological function summary

Cytohesin 1 influences multiple cellular processes like actin cytoskeleton remodeling cell migration and adhesion. It directs signal transduction pathways that regulate these key processes contributing to how cells interact with their external environment. Although Cytohesin 1 can function alone it typically operates as part of protein complexes that include partners like ARNO (another GEF for Arf proteins) that fine-tune intracellular signaling dynamics. This protein also interacts with other signaling molecules through its Sec7 and PH domains expanding its role across various cellular contexts.

Pathways

Cytohesin 1 integrates into significant signaling cascades such as the Arf6 pathway and the phosphoinositide 3-kinase (PI3K) pathway. Through these pathways it compounds cellular responses related to vesicular trafficking and cell morphology adjustments. In the Arf6 pathway Cytohesin 1 acts alongside proteins such as Arf6 and EFA6 to modulate membrane dynamics essential for endocytosis and stress fiber formation. In the PI3K pathway it collaborates with signaling molecules to regulate growth factor-mediated responses highlighting the interconnectivity Cytohesin 1 maintains between various pathway components.

Cytohesin 1 has been linked to immune-related diseases and neurological disorders. In the context of the immune system it plays a role in T cell activation and inflammation and alterations in its functioning may contribute to conditions like autoimmunity. In neurological contexts Cytohesin 1 interacts with proteins related to cellular adhesion and neurite outgrowth which can impact neurological functions and may play a role in developmental disorders. Its relationship with proteins like integrins also suggests how its dysregulation can affect cell attachment and signaling impacting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.
See full target information CYTH1
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Product promise

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For full details, please see our Terms & Conditions

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