AB151732

Anti-Cytohesin 1 antibody [EPR10051]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Cytohesin 1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

D17S811E, PSCD1, CYTH1, Cytohesin-1, SEC7 homolog B2-1

2 Images

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] (AB151732)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] (AB151732)

Intracellular flow cytometric analysis of permeabilized HUT78 cells labeling Cytohesin 1 using ab151732 at a 1/100 dilution (red) or a rabbit IgG control (negative) (green).

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Western blot - Anti-Cytohesin 1 antibody [EPR10051] (AB151732)

All lanes:

Western blot - Anti-Cytohesin 1 antibody [EPR10051] (ab151732) at 1/1000 dilution

Lane 1:

Jurkat cell lysates

Lane 2:

Raji cell lysates

Lane 3:

K562 cell lysates

Lane 4:

HUT78 cell lysates

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 46 kDa

false

Carrier free

Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10051

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytohesin 1 also known as CYTH1 or PSCD1 belongs to the cytohesin family. It plays a role as a guanine nucleotide exchange factor (GEF) specifically for Arf (ADP-ribosylation factor) proteins. Cytohesin 1 weighs approximately 47 kDa. It contributes to cellular communication and membrane trafficking activities by activating Arf family GTPases. You can find it widely expressed in tissues including immune cells and the nervous system. It localizes to cytoplasmic regions and associates with the plasma membrane through a pleckstrin homology (PH) domain.

Biological function summary

Cytohesin 1 influences multiple cellular processes like actin cytoskeleton remodeling cell migration and adhesion. It directs signal transduction pathways that regulate these key processes contributing to how cells interact with their external environment. Although Cytohesin 1 can function alone it typically operates as part of protein complexes that include partners like ARNO (another GEF for Arf proteins) that fine-tune intracellular signaling dynamics. This protein also interacts with other signaling molecules through its Sec7 and PH domains expanding its role across various cellular contexts.

Pathways

Cytohesin 1 integrates into significant signaling cascades such as the Arf6 pathway and the phosphoinositide 3-kinase (PI3K) pathway. Through these pathways it compounds cellular responses related to vesicular trafficking and cell morphology adjustments. In the Arf6 pathway Cytohesin 1 acts alongside proteins such as Arf6 and EFA6 to modulate membrane dynamics essential for endocytosis and stress fiber formation. In the PI3K pathway it collaborates with signaling molecules to regulate growth factor-mediated responses highlighting the interconnectivity Cytohesin 1 maintains between various pathway components.

Cytohesin 1 has been linked to immune-related diseases and neurological disorders. In the context of the immune system it plays a role in T cell activation and inflammation and alterations in its functioning may contribute to conditions like autoimmunity. In neurological contexts Cytohesin 1 interacts with proteins related to cellular adhesion and neurite outgrowth which can impact neurological functions and may play a role in developmental disorders. Its relationship with proteins like integrins also suggests how its dysregulation can affect cell attachment and signaling impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.

See full target information CYTH1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 14:158 PubMed34784955

2021

Curcumin inhibits ovarian cancer progression by regulating circ-PLEKHM3/miR-320a/SMG1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Sifan Sun,Hailiang Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytohesin 1 antibody [EPR10051]

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] (AB151732)

Western blot - Anti-Cytohesin 1 antibody [EPR10051] (AB151732)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise