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AB155691

Anti-Cytohesin 3 antibody

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(2 Publications)

Rabbit Polyclonal Cytohesin 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CYTH3 aa 1-200.

View Alternative Names

ARNO3, GRP1, PSCD3, CYTH3, Cytohesin-3, ARF nucleotide-binding site opener 3, General receptor of phosphoinositides 1, Protein ARNO3, Grp1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 3 antibody (AB155691)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 3 antibody (AB155691)

Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Cytohesin 3 with ab155691 at 1/200. Image at the bottom costained with Hoechst 33342.

Western blot - Anti-Cytohesin 3 antibody (AB155691)
  • WB

Unknown

Western blot - Anti-Cytohesin 3 antibody (AB155691)

12% SDS PAGE

All lanes:

Western blot - Anti-Cytohesin 3 antibody (ab155691) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CYTH3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O43739

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytohesin 3 also known as CYTH3 is a small GTPase regulator involved in cellular signal transduction. It weighs approximately 47 kDa and is primarily expressed in brain tissues and immune cells. CYTH3 functions as a guanine nucleotide exchange factor (GEF) for Arf family of small GTPases. Through its Sec7 domain it facilitates the exchange of GDP for GTP activating Arf proteins. Its pleckstrin homology (PH) domain aids in membrane association by binding phosphoinositides therefore localizing CYTH3 activity to cell membranes where signal transduction events occur.
Biological function summary

Signaling activities that require rapid membrane dynamics involve CYTH3. It participates in vesicular trafficking cell adhesion and cytoskeletal rearrangements. CYTH3 plays a role in processes such as endocytosis and exocytosis due to its interaction with Arf proteins involved in these pathways. Additionally it contributes to immune responses and cellular communication by being part of signaling complexes that respond to extracellular stimuli.

Pathways

CYTH3 acts as an important regulator in the Arf signaling cascade and also the PI3K/Akt pathway. In the Arf signaling pathway CYTH3 modulates vesicle formation and membrane trafficking. In the PI3K/Akt pathway CYTH3 interacts with phosphoinositides and impacts downstream signaling processes linked to cell survival growth and metabolism. Related proteins include Arf6 which functions in endocytosis and PI3K which is involved in many cellular pathways related to growth and survival.

The dysregulation of CYTH3 activity can contribute to the pathology of neurodegenerative diseases and immune system dysfunctions. Abnormal CYTH3 activity in neurons can affect synaptic vesicle trafficking which is related to diseases like Alzheimer’s. Additionally improper regulation in immune cells may shift cellular responses contributing to inflammation and autoimmune diseases. CYTH3's interactions with proteins such as Arf6 and PI3K emphasize its potential role in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays a role in the epithelial polarization (By similarity).
See full target information CYTH3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:876-890 PubMed40562875

2025

AIMP3 maintains cardiac homeostasis by regulating the editing activity of methionyl-tRNA synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Anindhya S Das,Charles P Rabolli,Colton R Martens,Han-Kai Jiang,Yingshen Zhang,Aubree A Zimmer,Kevin Lin,Kedryn K Baskin,Juan D Alfonzo,Federica Accornero

Nature communications 14:3560 PubMed37322019

2023

CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Serwe,David Kachaner,Jessica Gagnon,Cédric Plutoni,Driss Lajoie,Eloïse Duramé,Malha Sahmi,Damien Garrido,Martin Lefrançois,Geneviève Arseneault,Marc K Saba-El-Leil,Sylvain Meloche,Gregory Emery,Marc Therrien
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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