Rabbit Polyclonal Cytohesin 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYTH3 aa 1-200.
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Publications
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- Nature communications 14:35602023CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Serwe,David Kachaner,Jessica Gagnon,Cédric Plutoni,Driss Lajoie,Eloïse Duramé,Malha Sahmi,Damien Garrido,Martin Lefrançois,Geneviève Arseneault,Marc K Saba-El-Leil,Sylvain Meloche,Gregory Emery,Marc TherrienPubMed 37322019
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYTH3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O43739
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays a role in the epithelial polarization (By similarity).
Alternative names
ARNO3, GRP1, PSCD3, CYTH3, Cytohesin-3, ARF nucleotide-binding site opener 3, General receptor of phosphoinositides 1, Protein ARNO3, Grp1
Recommended products
Rabbit Polyclonal Cytohesin 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYTH3 aa 1-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYTH3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O43739
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytohesin 3 also known as CYTH3 is a small GTPase regulator involved in cellular signal transduction. It weighs approximately 47 kDa and is primarily expressed in brain tissues and immune cells. CYTH3 functions as a guanine nucleotide exchange factor (GEF) for Arf family of small GTPases. Through its Sec7 domain it facilitates the exchange of GDP for GTP activating Arf proteins. Its pleckstrin homology (PH) domain aids in membrane association by binding phosphoinositides therefore localizing CYTH3 activity to cell membranes where signal transduction events occur.
- Biological function summary
Signaling activities that require rapid membrane dynamics involve CYTH3. It participates in vesicular trafficking cell adhesion and cytoskeletal rearrangements. CYTH3 plays a role in processes such as endocytosis and exocytosis due to its interaction with Arf proteins involved in these pathways. Additionally it contributes to immune responses and cellular communication by being part of signaling complexes that respond to extracellular stimuli.
- Pathways
CYTH3 acts as an important regulator in the Arf signaling cascade and also the PI3K/Akt pathway. In the Arf signaling pathway CYTH3 modulates vesicle formation and membrane trafficking. In the PI3K/Akt pathway CYTH3 interacts with phosphoinositides and impacts downstream signaling processes linked to cell survival growth and metabolism. Related proteins include Arf6 which functions in endocytosis and PI3K which is involved in many cellular pathways related to growth and survival.
- Associated diseases and disorders
The dysregulation of CYTH3 activity can contribute to the pathology of neurodegenerative diseases and immune system dysfunctions. Abnormal CYTH3 activity in neurons can affect synaptic vesicle trafficking which is related to diseases like Alzheimer’s. Additionally improper regulation in immune cells may shift cellular responses contributing to inflammation and autoimmune diseases. CYTH3's interactions with proteins such as Arf6 and PI3K emphasize its potential role in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Cytohesin 3 antibody (ab155691)
12% SDS PAGE
All lanes: Western blot - Anti-Cytohesin 3 antibody (ab155691) at 1/1000 dilution
All lanes: NT2D1 whole cell lysate at 30 µg
Predicted band size: 46 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 3 antibody (ab155691)
Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Cytohesin 3 with ab155691 at 1/200. Image at the bottom costained with Hoechst 33342.
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