AB93652

Anti-Cytokeratin 1 antibody

Name: Anti-Cytokeratin 1 antibody (ab93652) | Abcam
Brand: Abcam Limited
SKU: AB93652
Price: 500 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(29 Publications)

Rabbit Polyclonal Cytokeratin 1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human KRT1.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (AB93652)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (AB93652)

ab93652, at a 1/100 dilution, staining Cytokeratin 1 in the membrane of formalin fixed, paraffin embedded skin tissue section by Immunohistochemistry.

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Western blot - Anti-Cytokeratin 1 antibody (AB93652)

All lanes:

Western blot - Anti-Cytokeratin 1 antibody (ab93652) at 1/500 dilution

All lanes:

fetal skin lysate

Predicted band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KRT1. The exact immunogen used to generate this antibody is proprietary information.

P04264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 1 also known as CK1 or Keratin 1 (with a molecular mass of approximately 66 kDa) functions as a structural protein integral to the keratinocyte cytoskeleton. Expressed mainly in the epidermis' suprabasal layers it plays an important role in maintaining the structural integrity of the skin. Its expression is identifiable through the use of anti-cytokeratin antibodies and anti-cytokeratin 1 antibodies which are specific tools for detecting cytokeratins in tissue sections.

Biological function summary

Cytokeratin 1 contributes to epithelial cell stability and resilience. It forms heterodimers with its partner keratin cytokeratin 10 and participates in creating a complex filament network that protects cells from mechanical stress. This network is essential for the proper functioning of the epithelial barrier which shields underlying tissues from physical damage and pathogen invasion.

Pathways

Cytokeratin 1 is a component of the dermal-epidermal junction integrity pathways and the differentiation pathway of keratinocytes. It interacts closely with cytokeratin 10 and other keratins which help mediate cellular responses to mechanical and chemical stresses. These interactions reinforce the signaling that guides keratinocyte differentiation and ensures the assembly of a well-organized epithelial layer.

Mutations or dysregulation in cytokeratin 1 can lead to skin disorders like ichthyosis hystrix and epidermolytic hyperkeratosis. These conditions typically involve disruptions in the structural framework of keratinocytes causing skin scaling and fragility. Abnormal interactions between cytokeratin 1 and proteins such as cytokeratin 10 are often implicated in these disorders highlighting their cooperative role in maintaining skin health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

See full target information KRT1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39682709

2024

Impairment of Intermediate Filament Expression Reveals Impact on Cell Functions Independent from Keratinocyte Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Klein,Imke Ramminger,Shuoqiu Bai,Thorsten Steinberg,Pascal Tomakidi

Nature communications 15:3366 PubMed38684678

2024

Skin graft with dermis and appendages generated in vivo by cell competition.

Applications

Unspecified application

Species

Unspecified reactive species

Hisato Nagano,Naoaki Mizuno,Hideyuki Sato,Eiji Mizutani,Ayaka Yanagida,Mayuko Kano,Mariko Kasai,Hiromi Yamamoto,Motoo Watanabe,Fabian Suchy,Hideki Masaki,Hiromitsu Nakauchi

Immunity, inflammation and disease 11:e1104 PubMed38156380

2024

Pro-inflammatory cytokine IL-6 regulates LMO4 expression in psoriatic keratinocytes via AKT/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Tu,Wei Wei,Qiantong Xiang,Wenwen Wang,Siping Zhang,Haisheng Zhou

JID innovations : skin science from molecules to population health 3:100175 PubMed36968096

2023

Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Elodie Henriet,Florence Abdallah,Yoan Laurent,Cyril Guimpied,Emily Clement,Michel Simon,Chantal Pichon,Patrick Baril

International journal of molecular sciences 24: PubMed37047109

2023

Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon Seo

Toxicon : official journal of the International Society on Toxinology 220:106955 PubMed36309071

2022

Proteomics and histological assessment of an organotypic model of human skin following exposure to Naja nigricollis venom.

Applications

Unspecified application

Species

Unspecified reactive species

Shirin Ahmadi,Spyridon T Pachis,Konstantinos Kalogeropoulos,Farrell McGeoghan,Vahap Canbay,Steven R Hall,Edouard P Crittenden,Charlotte A Dawson,Keirah E Bartlett,José María Gutiérrez,Nicholas R Casewell,Ulrich Auf dem Keller,Andreas H Laustsen

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

Cell regeneration (London, England) 10:36 PubMed34719766

2021

Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Zixian Zhao,Yujia Wang,Yingchuan Wu,Dandan Li,Ting Zhang,Yu Ma,Xiaoming Teng,Wei Zuo

Stem cells international 2021:5512153 PubMed34721590

2021

METTL3-Mediated mA RNA Modification Regulates Corneal Injury Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yarong Dai,Maosheng Cheng,Siyan Zhang,Rongsong Ling,Jieqi Wen,Yifan Cheng,Boxuan Huang,Jinrong Li,Caifeng Dai,Shiqing Mao,Shuibin Lin,Huangxuan Shen,Yizhou Jiang

International journal of molecular sciences 22: PubMed34575938

2021

FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Wang,Thorsten Steinberg,Martin P Dieterle,Imke Ramminger,Ayman Husari,Pascal Tomakidi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody (AB93652)

Western blot - Anti-Cytokeratin 1 antibody (AB93652)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

Product promise