Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)

Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Suitable for WB, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 17 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-Fr	IHC-P	Flow Cyt (Intra)	ICC/IF
Human	Tested	Expected	Tested	Tested	Tested
Mouse	Not recommended	Tested	Tested	Expected	Not recommended
Rat	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/20000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/20000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500 - 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Associated Products

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Target data

See full target information KRT1

Function

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Alternative names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Suitable for WB, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 17 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17744
Purification technique: Affinity purification Protein A
Specificity: Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 1 also known as CK1 or Keratin 1 (with a molecular mass of approximately 66 kDa) functions as a structural protein integral to the keratinocyte cytoskeleton. Expressed mainly in the epidermis' suprabasal layers it plays an important role in maintaining the structural integrity of the skin. Its expression is identifiable through the use of anti-cytokeratin antibodies and anti-cytokeratin 1 antibodies which are specific tools for detecting cytokeratins in tissue sections.

Biological function summary

Cytokeratin 1 contributes to epithelial cell stability and resilience. It forms heterodimers with its partner keratin cytokeratin 10 and participates in creating a complex filament network that protects cells from mechanical stress. This network is essential for the proper functioning of the epithelial barrier which shields underlying tissues from physical damage and pathogen invasion.

Pathways

Cytokeratin 1 is a component of the dermal-epidermal junction integrity pathways and the differentiation pathway of keratinocytes. It interacts closely with cytokeratin 10 and other keratins which help mediate cellular responses to mechanical and chemical stresses. These interactions reinforce the signaling that guides keratinocyte differentiation and ensures the assembly of a well-organized epithelial layer.

Associated diseases and disorders

Mutations or dysregulation in cytokeratin 1 can lead to skin disorders like ichthyosis hystrix and epidermolytic hyperkeratosis. These conditions typically involve disruptions in the structural framework of keratinocytes causing skin scaling and fragility. Abnormal interactions between cytokeratin 1 and proteins such as cytokeratin 10 are often implicated in these disorders highlighting their cooperative role in maintaining skin health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on mouse skin is observed (PMID: 12010363).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative Control: No staining on mouse stomach.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on rat skin is observed (PMID: 12010363).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative Control: No staining on rat kidney.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
The result showed cytoplasmic staining on mouse skin.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Blocking/Dilution buffer: 5% NFDM/TBST.
Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).
All lanes: Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution
Lane 1: Human skin lysate at 10 µg
Lane 2: Human fetal brain lysate at 10 µg
Lane 3: Human fetal heart lysate at 10 µg
Lane 4: Human fetal kidney lysate at 10 µg
Lane 5: Human spleen lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3min
Flow Cytometry (Intracellular) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) labelling Cytokeratin 1 with ab185628 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Cytokeratin 1 with ab185628 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution.
Confocal image showing cytoplasmic staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Cytoplasmic staining on human skin.

The section was incubated with ab185628 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
Immunohistochemical analysis of paraffin-embedded human kidney labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Negative Control: no staining on human kidney.

The section was incubated with ab185628 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.