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AB185628

Anti-Cytokeratin 1 antibody [EPR17744]

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(26 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Suitable for WB, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 26 publications.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Cytokeratin 1 with ab185628 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution.

Confocal image showing cytoplasmic staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded human kidney labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative Control : no staining on human kidney.
The section was incubated with ab185628 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) labelling Cytokeratin 1 with ab185628 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on human skin.
The section was incubated with ab185628 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control : No staining on mouse stomach.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control : No staining on rat kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat skin is observed (PMID : 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse skin.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse skin is observed (PMID : 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

Blocking/Dilution buffer : 5% NFDM/TBST.

Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID : 2580302).

All lanes:

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17744

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.

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Alternative Products

Primary Antibodies

AB9286

Anti-Epithelial Keratin Type I antibody [AE1]

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Primary Antibodies

AB93652

Anti-Cytokeratin 1 antibody

Western blot - Anti-Cytokeratin 1 antibody (AB93652)

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Primary Antibodies

AB194344

Anti-Cytokeratin 1 antibody

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Primary Antibodies

AB185629

Anti-Cytokeratin 1 antibody [EPR17870]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629)

  • 0
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Complementary Products

Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

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Primary Antibodies

AB76318

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

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Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 1 also known as CK1 or Keratin 1 (with a molecular mass of approximately 66 kDa) functions as a structural protein integral to the keratinocyte cytoskeleton. Expressed mainly in the epidermis' suprabasal layers it plays an important role in maintaining the structural integrity of the skin. Its expression is identifiable through the use of anti-cytokeratin antibodies and anti-cytokeratin 1 antibodies which are specific tools for detecting cytokeratins in tissue sections.
Biological function summary

Cytokeratin 1 contributes to epithelial cell stability and resilience. It forms heterodimers with its partner keratin cytokeratin 10 and participates in creating a complex filament network that protects cells from mechanical stress. This network is essential for the proper functioning of the epithelial barrier which shields underlying tissues from physical damage and pathogen invasion.

Pathways

Cytokeratin 1 is a component of the dermal-epidermal junction integrity pathways and the differentiation pathway of keratinocytes. It interacts closely with cytokeratin 10 and other keratins which help mediate cellular responses to mechanical and chemical stresses. These interactions reinforce the signaling that guides keratinocyte differentiation and ensures the assembly of a well-organized epithelial layer.

Mutations or dysregulation in cytokeratin 1 can lead to skin disorders like ichthyosis hystrix and epidermolytic hyperkeratosis. These conditions typically involve disruptions in the structural framework of keratinocytes causing skin scaling and fragility. Abnormal interactions between cytokeratin 1 and proteins such as cytokeratin 10 are often implicated in these disorders highlighting their cooperative role in maintaining skin health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
See full target information KRT1
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26798 PubMed40702080

2025

Decreased green autofluorescence in cancerous tissues is a potential biomarker for diagnosis of renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wan,Junrong Zou,Tianpeng Xie,Liang Zeng,Huiquan Liu,Bo Jiang,Yunfeng Liao,Yuting Wu,Gengqing Wu,Guoxi Zhang,Weihai Ying,Xiaofeng Zou

Journal of nanobiotechnology 23:506 PubMed40660260

2025

Human adipose-derived mesenchymal stem cell-derived exosomes induce epithelial remodeling and anti-scar healing revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Jun-Ling Xie,Xing-Liao Zhang,Guang-Ming Xie,Xin-Min Zhang,Yao-Ting Han,Meng-Meng Xu,Jing Zhang,Jun Zhang

Scientific reports 15:6377 PubMed39984626

2025

Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Malak,Chen Qian,Jeemol James,Syam Nair,Julie Grantham,Marica B Ericson

Experimental and therapeutic medicine 28:402 PubMed39234585

2024

PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Xia,Jin Li,Hongshan Yuan,Wenliang Yan

Investigative ophthalmology & visual science 65:8 PubMed38315493

2024

Urban Particulate Matter Triggers Meibomian Gland Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqian Tu,Ren Liu,Jianwen Xue,Bing Xiao,Jing Li,Lingyi Liang

Investigative ophthalmology & visual science 64:29 PubMed38133507

2023

Isolation and Culture of Human Meibomian Gland Ductal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Peng,Ya-Li Du,Shu-Ting Liu,Hua Chen,Jia-Song Wang,Chao Wang,Hua-Tao Xie,Ming-Chang Zhang

Scientific reports 13:12261 PubMed37507439

2023

Full thickness 3D in vitro conjunctiva model enables goblet cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Schwebler,Christina Fey,Daniel Kampik,Christian Lotz

Molecular pharmaceutics 20:3570-3577 PubMed37307328

2023

Peptide-Drug Conjugate Targeting Keratin 1 Inhibits Triple-Negative Breast Cancer in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elmira Ziaei,Igor Moura de Paiva,Shih-Jing Yao,Nasim Sarrami,Parnian Mehinrad,Justine Lai,Afsaneh Lavasanifar,Kamaljit Kaur

Investigative ophthalmology & visual science 64:19 PubMed36692471

2023

Effect of TRPM8 Functional Loss on Corneal Epithelial Wound Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Ran,Jing Feng,Xia Qi,Ting Liu,Benxiang Qi,Kai Jiang,Zhenzhen Zhang,Yang Yu,Qingjun Zhou,Lixin Xie

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22776 PubMed36688817

2023

Loss of aquaporin 5 contributes to the corneal epithelial pathogenesis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Guohu Di,Kaier Zhang,Ying Bai,Xin Cao,Hui Zhao,Dianqiang Wang,Peng Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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