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AB250844

Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human, Rat, Mouse samples.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Cytokeratin 1 with ab185628 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution.

Confocal image showing cytoplasmic staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative Control : no staining on human kidney.
The section was incubated with ab185628 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (human epidermoid carcinoma epithelial cell) labelling Cytokeratin 1 with ab185628 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 1 with ab185628 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on human skin.
The section was incubated with ab185628 for 15 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control : No staining on mouse stomach.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative Control : No staining on rat kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat skin is observed (PMID : 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse skin.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse skin is observed (PMID : 12010363).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (AB250844)

This data was developed using ab185628, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID : 2580302).

All lanes:

Western blot - Anti-Cytokeratin 1 antibody [EPR17744] (<a href='/en-us/products/primary-antibodies/cytokeratin-1-antibody-epr17744-ab185628'>ab185628</a>) at 1/20000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17744

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.

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Reactivity data

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Product details

ab250844 is the carrier-free version of ab185628.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 1 also known as CK1 or Keratin 1 (with a molecular mass of approximately 66 kDa) functions as a structural protein integral to the keratinocyte cytoskeleton. Expressed mainly in the epidermis' suprabasal layers it plays an important role in maintaining the structural integrity of the skin. Its expression is identifiable through the use of anti-cytokeratin antibodies and anti-cytokeratin 1 antibodies which are specific tools for detecting cytokeratins in tissue sections.
Biological function summary

Cytokeratin 1 contributes to epithelial cell stability and resilience. It forms heterodimers with its partner keratin cytokeratin 10 and participates in creating a complex filament network that protects cells from mechanical stress. This network is essential for the proper functioning of the epithelial barrier which shields underlying tissues from physical damage and pathogen invasion.

Pathways

Cytokeratin 1 is a component of the dermal-epidermal junction integrity pathways and the differentiation pathway of keratinocytes. It interacts closely with cytokeratin 10 and other keratins which help mediate cellular responses to mechanical and chemical stresses. These interactions reinforce the signaling that guides keratinocyte differentiation and ensures the assembly of a well-organized epithelial layer.

Mutations or dysregulation in cytokeratin 1 can lead to skin disorders like ichthyosis hystrix and epidermolytic hyperkeratosis. These conditions typically involve disruptions in the structural framework of keratinocytes causing skin scaling and fragility. Abnormal interactions between cytokeratin 1 and proteins such as cytokeratin 10 are often implicated in these disorders highlighting their cooperative role in maintaining skin health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
See full target information KRT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com