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Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 5 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629), expandable thumbnail
  • Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (AB185629), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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4 products for Alternative Product

Target data

Function

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK (By similarity).

Alternative names

Krt2-1, Krt1, 67 kDa cytokeratin, Cytokeratin-1, Keratin-1, Type-II keratin Kb1, CK-1, K1

Recommended products

Rabbit Recombinant Monoclonal Cytokeratin 1 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17870
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 1 also known as CK1 or Keratin 1 (with a molecular mass of approximately 66 kDa) functions as a structural protein integral to the keratinocyte cytoskeleton. Expressed mainly in the epidermis' suprabasal layers it plays an important role in maintaining the structural integrity of the skin. Its expression is identifiable through the use of anti-cytokeratin antibodies and anti-cytokeratin 1 antibodies which are specific tools for detecting cytokeratins in tissue sections.

Biological function summary

Cytokeratin 1 contributes to epithelial cell stability and resilience. It forms heterodimers with its partner keratin cytokeratin 10 and participates in creating a complex filament network that protects cells from mechanical stress. This network is essential for the proper functioning of the epithelial barrier which shields underlying tissues from physical damage and pathogen invasion.

Pathways

Cytokeratin 1 is a component of the dermal-epidermal junction integrity pathways and the differentiation pathway of keratinocytes. It interacts closely with cytokeratin 10 and other keratins which help mediate cellular responses to mechanical and chemical stresses. These interactions reinforce the signaling that guides keratinocyte differentiation and ensures the assembly of a well-organized epithelial layer.

Associated diseases and disorders

Mutations or dysregulation in cytokeratin 1 can lead to skin disorders like ichthyosis hystrix and epidermolytic hyperkeratosis. These conditions typically involve disruptions in the structural framework of keratinocytes causing skin scaling and fragility. Abnormal interactions between cytokeratin 1 and proteins such as cytokeratin 10 are often implicated in these disorders highlighting their cooperative role in maintaining skin health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on keratinized epithelium of the mouse skin tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed frozen mouse skin tissue labeling Cytokeratin 1 with ab185629 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution. Confocal image showing cytoplasm staining on keratinized epithelium of the mouse skin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.

  • Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629), expandable thumbnail

    Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Cytokeratin 1 is expressed in terminally differentiating epidermis.

    All lanes: Western blot - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629) at 1/5000 dilution

    Lane 1: Mouse skin tissue lysate at 10 µg

    Lane 2: Mouse brain tissue lysate at 10 µg

    Lane 3: Mouse heart tissue lysate at 10 µg

    Lane 4: Mouse kidney tissue lysate at 10 µg

    Lane 5: Mouse spleen tissue lysate at 10 µg

    Lane 6: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 7: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa, 44 kDa, 66 kDa

    Observed band size: 21 kDa, 32 kDa, 45 kDa, 66 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17870] (ab185629)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Cytokeratin 1 with ab185629 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Negative on mouse brain tissue. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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