Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91]

8 product images

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  Multiplex immunohistochemistry - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skin staining of Cytokeratin 1 + Cytokeratin 2e with ab315228 at a 1/2000 (0.263 μg/ml) dilution, Collagen I with Anti-Collagen I antibody [EPR24331-53] ab270993 and Langerin with Anti-Langerin antibody [EPR24685-12] ab283686 at a 1/1000 dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-Cytokeratin 1 + Cytokeratin 2e (green; Opal™520), anti-Collagen I (magenta; Opal™690) and anti-Langerin (gray; Opal™570) on mouse skin.
  
  Panel B: anti-Cytokeratin 1 + Cytokeratin 2e staining epidermis keratin layer in mouse skin.
  
  Panel C: anti-Collagen I staining connective tissues in mouse skin.
  
  Panel D: anti-Langerin staining Langerhans cells in mouse skin.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of ab315228, Anti-Collagen I antibody [EPR24331-53] ab270993 and Anti-Langerin antibody [EPR24685-12] ab283686 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system,

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/100 (5.25 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

  Negative control: Confocal image showing no staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315228 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin (fresh frozen) tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/100 (5.25 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing positive staining on mouse skin. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315228 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse cerebrum. The section was incubated with ab315228 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on human cerebrum. The section was incubated with ab315228 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse skin epidermis. The section was incubated with ab315228 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 1 + Cytokeratin 2e with ab315228 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human skin epidermis (PMID: 33441834). The section was incubated with ab315228 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Dot Blot - Anti-Cytokeratin 1 + Cytokeratin 2e antibody [EPR27257-91] (ab315228)

  Dot blot analysis of Cytokeratin 1 + Cytokeratin 2e using ab315228 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

  Lane 1: His-tagged human KRT1 recombinant protein

  Lane 2: Purified His-tagged human KRT2 recombinant protein

  Exposure time: 180 seconds.

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.