Anti-Cytokeratin 10 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody (AB111447)

IHC image of Cytokeratin 10 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab111447, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

AbReview32707****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody (AB111447)

IHC-P image of Cytokeratin 10 staining using human skin using ab111447 (1 : 6000). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab111447 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1 : 250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody (AB111447)

ab111447 staining Cytokeratin 10 in A-431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab111447 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

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Western blot - Anti-Cytokeratin 10 antibody (AB111447)

All lanes:

Western blot - Anti-Cytokeratin 10 antibody (ab111447) at 1 µg/mL

All lanes:

Human skin tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skin-tissue-lysate-total-protein-ab30166'>ab30166</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

true

Exposure time: 16min

• IHC-P

AbReview33017****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody (AB111447)

IHC-P image of Cytokeratin 10 staining using rat E18 skin using ab111447 (1 : 5000). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab111447 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1 : 250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom